Fialkow P J, Denman A M, Jacobson R J, Lowenthal M N
J Clin Invest. 1978 Oct;62(4):815-23. doi: 10.1172/JCI109193.
In three patients with chronic myelocytic leukemia who were heterozygous at the X-linked glucose-6-phospháte dehydrogenase locus, lymphocytes were studied to determine if they had the same stem cell origin as the leukemic myeloid cells. Normal tissues such as skin had both B and A glucose-6-phosphate dehydrogenase isoenzymes, but the leukemic myelogenous cells displayed only one isoenzyme type, consistent with their clonal origin. A population of cells with undoubted thymus-derived (T)-lymphocyte characteristics had both isoenzymes. Presumably, then, these T cells did not arise from the leukemic stem cell, either because they antedated the development of leukemia in that stem cell or, more likely, because they arose from progenitors not involved by the disease. In contrast, another population of lymphocytes showed only one isoenzyme type, suggesting that it arose from the chronic myelocytic leukemia stem cell. However, although this population contained many cells with the characteristics of bone marrow-derived (B) lymphocytes, it is not certain that the single enzyme produced by the cells over all can be attributed to B lymphocytes rather than to contaminating non-B-lymphoid cells.
在3名X连锁葡萄糖-6-磷酸脱氢酶位点为杂合子的慢性粒细胞白血病患者中，对淋巴细胞进行了研究，以确定它们是否与白血病髓细胞具有相同的干细胞起源。正常组织如皮肤同时具有B型和A型葡萄糖-6-磷酸脱氢酶同工酶，但白血病髓细胞仅表现出一种同工酶类型，这与其克隆起源一致。一群具有明确胸腺来源（T）淋巴细胞特征的细胞同时具有两种同工酶。那么，推测这些T细胞并非来自白血病干细胞，要么是因为它们在该干细胞发生白血病之前就已存在，要么更有可能是因为它们起源于未受该疾病影响的祖细胞。相比之下，另一群淋巴细胞仅表现出一种同工酶类型，提示其起源于慢性粒细胞白血病干细胞。然而，尽管这群细胞包含许多具有骨髓来源（B）淋巴细胞特征的细胞，但尚不能确定细胞总体产生的单一酶可归因于B淋巴细胞而非污染的非B淋巴细胞。
