Durántez A, de Landazuri M O, Silva A, Alvarez J, Kreisler M
Department of Internal Medicine I, Clinica Puerta de Hierro, I.N.P. Universidad Autónoma, Madrid, Spain.
J Clin Lab Immunol. 1979 Apr;2(1):59-65.
Normal human lymphoid cells from peripheral blood, spleen, tonsils and thymus were examined for their ability to mediate three different cytotoxic effector cell functions: antibody-dependent cellular cytotoxicity (ADCC); lectin-induced cellular cytotoxicity (LICC) and natural killer activity (NK), against 51Cr labelled erythroid and tumor target cells. We found a hierarchy of cytotoxic activities in the different lymphoid tissues. Peripheral blood and spleen cells were able to mediate LICC, ADCC and NK activities. Tonsil cells showed a natural segregation of the different cytotoxic functions: NK and ADCC activity against tumor target cells were absent, whereas LICC activity was fully present. With respect to ADCC activity against erythroid targets, tonsil cells showed low, but significant, cytotoxicity. Thymus cells had no detectable ADCC, NK and LICC activities. Correlation in the different lymphoid tissues between cytotoxic activities and cell surface marker studies revealed: (a) that the presence of E-SRBC rosette forming cells was not always associated with the detection of LICC activity, as is the case with the thymus; (b) that, in the absence of detectable Eox-7S rosette forming cells (thymus and tonsils), NK and ADCC activities against tumor cells were always absent, but LICC was observed (tonsils), indicating that the presence of this Fc(7S) receptor bearing cells is strongly associated with the expression of NK and ADCC but not with LICC.
对来自外周血、脾脏、扁桃体和胸腺的正常人淋巴细胞进行检测，以评估它们介导三种不同细胞毒性效应细胞功能的能力：抗体依赖性细胞毒性（ADCC）、凝集素诱导的细胞毒性（LICC）和自然杀伤活性（NK），检测对象为用51Cr标记的红细胞和肿瘤靶细胞。我们发现不同淋巴组织中的细胞毒性活性存在层级关系。外周血和脾细胞能够介导LICC、ADCC和NK活性。扁桃体细胞表现出不同细胞毒性功能的自然分离：对肿瘤靶细胞不存在NK和ADCC活性，而LICC活性完全存在。关于对红细胞靶标的ADCC活性，扁桃体细胞表现出低但显著的细胞毒性。胸腺细胞没有可检测到的ADCC、NK和LICC活性。不同淋巴组织中细胞毒性活性与细胞表面标志物研究之间的相关性表明：（a）E-SRBC花环形成细胞的存在并不总是与LICC活性的检测相关，如胸腺的情况；（b）在没有可检测到的Eox-7S花环形成细胞（胸腺和扁桃体）的情况下，对肿瘤细胞的NK和ADCC活性总是不存在，但观察到了LICC活性（扁桃体），这表明这种携带Fc(7S)受体的细胞的存在与NK和ADCC的表达密切相关，但与LICC无关。
