Department of Ophthalmology, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning 110032, P.R. China.
Department of Ophthalmology, Shenyang Fourth People's Hospital, Shenyang, Liaoning 110031, P.R. China.
Mol Med Rep. 2019 May;19(5):4011-4018. doi: 10.3892/mmr.2019.10068. Epub 2019 Mar 21.
The aim of the present study was to confirm the effect of 2% rebamipide ophthalmic solution on a scopolamine‑induced dry eye (DE) mouse model, and to investigate its effect on the ratio of T helper cell 17 (Th17)/regulatory T cell (Treg) numbers. C57BL/6 mice received subcutaneous injections of scopolamine and were exposed to a low‑humidity environment in order to establish a DE model. Rebamipide eye drops (2%) administered four times daily for 2 weeks, significantly reduced the corneal staining scores and increased the tear film breakup time and tear production in the DE mice. Pathologically, the rebamipide restored the histological changes induced by DE in the cornea, conjunctiva and lacrimal gland. At a molecular level, it downregulated pro‑inflammatory and upregulated anti‑inflammatory cytokines in the conjunctiva and lacrimal gland. Furthermore, the increased Th17 and Treg levels were restored following treatment with rebamipide. In conclusion, the anti‑inflammatory and Th17/Treg balance‑preserving effects of rebamipide may contribute to the treatment of DE in mice.
本研究旨在证实 2%瑞巴派特滴眼液对东莨菪碱诱导的干眼症（DE）小鼠模型的作用，并研究其对辅助性 T 细胞 17（Th17）/调节性 T 细胞（Treg）比值的影响。C57BL/6 小鼠接受东莨菪碱皮下注射，并暴露于低湿度环境中以建立 DE 模型。瑞巴派特滴眼液（2%）每日滴眼 4 次，连续 2 周，可显著降低角膜染色评分，并增加 DE 小鼠的泪膜破裂时间和泪液分泌。在病理上，瑞巴派特恢复了 DE 引起的角膜、结膜和泪腺的组织学变化。在分子水平上，它下调了结膜和泪腺中的促炎细胞因子，上调了抗炎细胞因子。此外，瑞巴派特治疗后可恢复升高的 Th17 和 Treg 水平。总之，瑞巴派特的抗炎和 Th17/Treg 平衡维持作用可能有助于治疗小鼠的 DE。
