Department of Ophthalmology, Keio University School of Medicine, Tokyo 1608582, Japan.
Department of Ophthalmology, Mugla Sitki Kocman University School of Medicine, Mugla 48000, Turkey.
Int J Mol Sci. 2019 Aug 18;20(16):4031. doi: 10.3390/ijms20164031.
Rebamipide ophthalmic solution is a mucin secretagogue which is an important therapeutic agent in the treatment of dry eye. It has been noted that dry eye in office workers is associated with a decrease in secretory mucin. This study aimed to evaluate the effects of 2% rebamipide ophthalmic solution in mice subjected to environmental dry eye stress (EDES), which mimics the conditions of office workers. Thirty eyes from thirty BALB/c mice (eight-week-old males) were divided into three treatment groups: artificial tear (vehicle), 2% rebamipide ophthalmic solution, and 0.1% hyaluronic acid (HA) ophthalmic solution. After four days of pretreatment, mice were exposed to EDES for three days. The corneal subbasal nerve and inflammatory cells were then examined using in vivo confocal microscopy. Following EDES exposure, the lissamine green staining score was significantly lower and corneal sensitivity was more preserved in the 2% rebamipide group than in the HA group. In addition, the subbasal nerve fiber density was significantly higher and the DC density was significantly lower in the 2% rebamipide group than in the HA group. Overall, the topical rebamipide ophthalmic solution showed more favorable therapeutic effects when compared to the HA ophthalmic solution in a mouse model of EDES, likely owing to its anti-inflammatory and neuroprotective effects.
贝前列素钠滴眼液是一种黏蛋白分泌促进剂，是治疗干眼症的重要治疗药物。据报道，上班族的干眼症与分泌性黏蛋白减少有关。本研究旨在评估 2%贝前列素钠滴眼液对模拟上班族环境干燥眼应激（EDES）的小鼠的影响。将 30 只 BALB/c 小鼠（8 周龄雄性）的 30 只眼睛分为三组：人工泪液（载体）、2%贝前列素钠滴眼液和 0.1%透明质酸（HA）滴眼液。预处理 4 天后，将小鼠暴露于 EDES 中 3 天。然后使用活体共聚焦显微镜检查角膜基底神经和炎症细胞。EDES 暴露后，2%贝前列素钠组的丽春红染色评分明显降低，角膜敏感性较 HA 组得到更好的保持。此外，2%贝前列素钠组的基底神经纤维密度明显高于 HA 组，DC 密度明显低于 HA 组。总的来说，与 HA 滴眼液相比，局部贝前列素钠滴眼液在 EDES 小鼠模型中显示出更有利的治疗效果，这可能与其抗炎和神经保护作用有关。
