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肝移植中生物能量和线粒体功能的评估。

Evaluation of bioenergetic and mitochondrial function in liver transplantation.

机构信息

Department of Surgery, Portuguese Oncology Institute, Coimbra, Portugal.

Department of Life Sciences, Faculty of Sciences and Technology, University of Coimbra, Coimbra, Portugal.

出版信息

Clin Mol Hepatol. 2019 Jun;25(2):190-198. doi: 10.3350/cmh.2018.0087. Epub 2019 Mar 22.

DOI:10.3350/cmh.2018.0087
PMID:30897898
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6589847/
Abstract

BACKGROUND/AIMS: We measured changes in mitochondrial function and bioenergetics that occur during ischemia/ reperfusion in fresh liver samples of patients undergoing liver transplantation. These variations correlated with markers of liver function and clinical outcome. Ischemia/reperfusion injury related to liver transplantation affects mitochondrial function and bioenergetics. Experimental studies were conducted to identify the role of bioenergetics and mitochondrial dysfunction. To the best of our knowledge, no investigation of these two factors' impacts on liver transplantation has been performed.

METHODS

This was a prospective study of 28 patients who underwent liver transplantation. We measured parameters of mitochondrial function and bioenergetics in biopsies performed during the procedure.

RESULTS

We observed a statistically significant reduction in mitochondrial membrane potential, an increase in lag phase, and decreases in mitochondrial respiration and adenosine triphosphate content (P<0.010). Higher postoperative aminotransferase peaks correlated with worse mitochondrial function; mitochondrial respiration correlated with arterial lactate (P<0.010).

CONCLUSION

There is a relationship between mitochondrial function and ischemia/reperfusion injury. The future use of these clinical markers as prognostic factors may allow early identification of post-transplant liver failure and may indicate the need to perform a new transplant.

摘要

背景/目的：我们测量了肝移植患者新鲜肝脏样本在缺血/再灌注过程中发生的线粒体功能和生物能量变化。这些变化与肝功能标志物和临床结果相关。与肝移植相关的缺血/再灌注损伤会影响线粒体功能和生物能量。已经进行了实验研究以确定生物能量和线粒体功能障碍的作用。据我们所知，尚未对这两个因素对肝移植的影响进行调查。

方法

这是一项对 28 例接受肝移植的患者进行的前瞻性研究。我们测量了手术过程中进行的活检的线粒体功能和生物能量参数。

结果

我们观察到线粒体膜电位显著降低，滞后期增加，线粒体呼吸和三磷酸腺苷含量降低（P<0.010）。术后转氨酶峰值较高与线粒体功能较差相关；线粒体呼吸与动脉乳酸呈正相关（P<0.010）。

结论

线粒体功能与缺血/再灌注损伤之间存在关系。这些临床标志物作为预后因素的未来应用可能允许早期识别肝移植后衰竭，并可能表明需要进行新的移植。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5405/6589847/5ff7dbc0f39d/cmh-2018-0087f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5405/6589847/d2c323bfbabc/cmh-2018-0087f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5405/6589847/cb6dd4d5186e/cmh-2018-0087f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5405/6589847/7630698b54f5/cmh-2018-0087f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5405/6589847/2f8dd75f1307/cmh-2018-0087f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5405/6589847/5ff7dbc0f39d/cmh-2018-0087f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5405/6589847/d2c323bfbabc/cmh-2018-0087f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5405/6589847/cb6dd4d5186e/cmh-2018-0087f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5405/6589847/7630698b54f5/cmh-2018-0087f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5405/6589847/2f8dd75f1307/cmh-2018-0087f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5405/6589847/5ff7dbc0f39d/cmh-2018-0087f5.jpg

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