Department of Orthopedics, People's Hospital of YingDe City Guangdong Province, Yingde City, China.
Department of Traumatology, Guangdong Second Provincial General Hospital, Guangzhou, China.
J Invest Surg. 2021 Jan;34(1):107-117. doi: 10.1080/08941939.2019.1584255. Epub 2019 Mar 21.
: Asiaticoside (AS) is extracted from the traditional herbal medicine , and has angiogenic, antioxidant, anti-inflammatory, and wound-healing effects. We investigated the effects of AS on skin flap survival. : Dorsal McFarlane flaps were harvested from 36 rats and divided into two groups: an experimental group treated with 40 mg/kg AS administered orally once daily, and a control group administered normal saline in an identical manner. On day 2, superoxide dismutase (SOD) and malondialdehyde (MDA) levels, and production of the cytokines tumor necrosis factor-α and interleukin (IL)-6 were evaluated. On day 7, tissue slices were stained with hematoxylin and eosin. The expression of vascular endothelial growth factor (VEGF), IL-6, and IL-1β were immunohistochemically evaluated. Microcirculatory flow was measured using laser Doppler flowmetry. Flap angiography, using the lead oxide-gelatin injection technique, was performed with the aid of a soft X-ray machine. : The AS group exhibited greater mean flap survival area, improved microcirculatory flow, and higher expression levels of SOD and VEGF compared with the control group. However, MDA levels and the inflammatory response were significantly reduced. : AS exhibits promise as a therapeutic option due to its effects on the viability and function of random skin flaps in rats.
: 积雪草苷（AS）从传统草药中提取，具有血管生成、抗氧化、抗炎和伤口愈合作用。我们研究了 AS 对皮瓣存活的影响。 : 从 36 只大鼠背部采集 McFarlane 皮瓣，分为两组：实验组给予 40mg/kg AS 口服，每日一次；对照组给予等量生理盐水。第 2 天，评估超氧化物歧化酶（SOD）和丙二醛（MDA）水平以及肿瘤坏死因子-α和白细胞介素（IL）-6 的产生。第 7 天，用苏木精和伊红染色组织切片。用免疫组化评估血管内皮生长因子（VEGF）、IL-6 和 IL-1β的表达。用激光多普勒血流仪测量微循环流量。使用软 X 射线机进行氧化铅-明胶注射技术的皮瓣血管造影。 : AS 组的平均皮瓣存活面积较大，微循环流量改善，SOD 和 VEGF 的表达水平较高，而 MDA 水平和炎症反应明显降低。 : AS 对大鼠随意皮瓣的存活和功能有积极作用，有望成为一种治疗选择。
