Detection of carbapenemase-producing Pseudomonas aeruginosa: Evaluation of the carbapenem inactivation method (CIM).
作者信息
Gutiérrez Sergio, Correa Adriana, Hernández-Gómez Cristhian, De La Cadena Elsa, Pallares Christian, Villegas María Virginia
机构信息
Bacterial Resistance and Hospital Epidemiology Unit, International Center for Medical Research and Training (CIDEIM), Cali, Colombia.
Bacterial Resistance and Hospital Epidemiology Unit, International Center for Medical Research and Training (CIDEIM), Cali, Colombia; Universidad Santiago de Cali, Cali, Colombia.
出版信息
Enferm Infecc Microbiol Clin (Engl Ed). 2019 Dec;37(10):648-651. doi: 10.1016/j.eimc.2019.02.004. Epub 2019 Mar 18.
INTRODUCTION
The carbapenem inactivation method (CIM) is a cost-effective assay for detecting carbapenemases. However, its interpretation is unclear for Pseudomonas spp. We evaluate its accuracy when meropenem is changed to imipenem.
METHODS
We analyzed 266 P. aeruginosa isolates. The CIM method consists of: resuspend bacterial colonies (a full 10μL loop) in 400μL water, in which a 10μg disk of meropenem/imipenem is immersed. After 2h of incubation (35°C), remove the disk, place it onto a Mueller-Hinton agar plate previously inoculated with Escherichia coli (ATCC 25922), and incubate at 35 ̊C between 18-24 h. Interpretation criteria (mm of inhibition zone): ≤19mm, positive; ≥25mm negative; 20-24mm, undetermined.
RESULTS
Imipenem improves the sensitivity and specificity of CIM when compared to meropenem (99.4% and 98.9%, vs. 91.9% and 94.7%, respectively).
CONCLUSIONS
The accuracy of CIM for carbapenemase detection in P. aeruginosa is increased with the use of imipenem.
Zotero 插件