一种改良的碳青霉烯失活方法 CIMTrisII，用于检测革兰氏阴性病原体产生的碳青霉烯酶。
An improved carbapenem inactivation method, CIMTrisII, for carbapenemase production by Gram-negative pathogens.
机构信息
1Department of Infectious, Respiratory, and Digestive Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan.
2Division of Clinical Laboratory and Blood Transfusion, University of the Ryukyus Hospital, Okinawa, Japan.
出版信息
J Med Microbiol. 2019 Feb;68(2):124-131. doi: 10.1099/jmm.0.000888. Epub 2018 Dec 10.
The modified carbapenem inactivation method (mCIM) is a simple phenotypic screening method for detecting carbapenemase production by Enterobacteriaceae and Pseudomonas aeruginosa. We recently developed another modified carbapenem inactivation method (CIMTris), in which carbapenemase is extracted from bacteria with Tris-HCl buffer, to detect carbapenemase production by Acinetobacter and Pseudomonas species. This study describes an improved carbapenem inactivation method, CIMTrisII, for detecting carbapenemase production by Gram-negative pathogens, including Enterobacteriaceae, Acinetobacter and Pseudomonas species. CIMTrisII was different from CIMTris in the concentration of Meropenem disks (5-µg MEM disks vs. 10-µg MEM disks), the inoculum volume of the bacteria (a 5-µl loopful vs. a 10 µl loopful) and the incubation time (1 vs. 2 h). CIMTrisII showed an overall sensitivity of 99.3 % and an overall specificity of 95.0 % for tested isolates. In comparison, CIMTris showed a sensitivity of 96.1 % and a specificity of 96.3 %, and mCIM showed a sensitivity of 67.1 % and a specificity of 100 %. CIMTrisII is thus deemed useful for detecting carbapenemase production by Gram-negative pathogens.
改良碳青霉烯失活法（mCIM）是一种用于检测肠杆菌科和铜绿假单胞菌产生碳青霉烯酶的简单表型筛选方法。我们最近开发了另一种改良碳青霉烯失活法（CIMTris），用 Tris-HCl 缓冲液从细菌中提取碳青霉烯酶，以检测不动杆菌和假单胞菌属产生的碳青霉烯酶。本研究描述了一种改良的碳青霉烯失活法（CIMTrisII），用于检测包括肠杆菌科、不动杆菌和假单胞菌属在内的革兰氏阴性病原体产生的碳青霉烯酶。CIMTrisII 与 CIMTris 的区别在于美罗培南纸片的浓度（5-µg MEM 纸片与 10-µg MEM 纸片）、细菌接种量（5-µl 接种环与 10-µl 接种环）和孵育时间（1 小时与 2 小时）。CIMTrisII 对测试分离株的总敏感性为 99.3%，总特异性为 95.0%。相比之下，CIMTris 的敏感性为 96.1%，特异性为 96.3%，mCIM 的敏感性为 67.1%，特异性为 100%。因此，CIMTrisII 被认为可用于检测革兰氏阴性病原体产生的碳青霉烯酶。
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