表面增强拉曼光谱法分析菌株中的变量选择与生物标志物相关性
VARIABLE SELECTION AND BIOMARKER CORRELATION IN THE ANALYSIS OF STRAINS BY SURFACE-ENHANCED RAMAN SPECTROSCOPY.
作者信息
Krause Duncan C, Hennigan Suzanne L, Henderson Kelley C, Clark Harrison J, Dluhy Richard A
机构信息
Department of Microbiology, University of Georgia, Athens, GA, USA.
Department of Chemistry, University of Alabama at Birmingham, Birmingham, AL, USA.
出版信息
Anal Lett. 2017;50(15):2412-2425. doi: 10.1080/00032719.2017.1287713. Epub 2017 Sep 12.
is a human respiratory tract pathogen causing chronic bronchitis and atypical or "walking" pneumonia. The major surface protein P1 must form complexes with proteins P30 and P40/P90 in order to function in receptor binding and gliding motility, and variability in P1 and P40/P90 distinguishes the two major genotypes. Strains belonging to each genotype can be differentiated with high sensitivity and specificity by utilizing surface-enhanced Raman spectroscopy on silver nanorod arrays. Here we used the variable selection method of Variable Importance in Projection (VIP) to identify Raman bands important in strain classification. Furthermore, VIP analysis of mutants lacking P40/P90, or P1and P40/P90, correlated certain Raman bands important in distinguishing genotypes, with specific mycoplasma surface protein composition and presentation. Variable selection, and its correlation with specific mycoplasma surface components, is an important next step in developing this platform for detection and genotyping.
是一种引起慢性支气管炎和非典型或“迁延性”肺炎的人类呼吸道病原体。主要表面蛋白P1必须与蛋白P30和P40/P90形成复合物，才能在受体结合和滑行运动中发挥作用，P1和P40/P90的变异性区分了两种主要基因型。通过在银纳米棒阵列上利用表面增强拉曼光谱，可高灵敏度和特异性地区分属于每种基因型的菌株。在这里，我们使用投影变量重要性（VIP）的变量选择方法来识别在菌株分类中重要的拉曼谱带。此外，对缺乏P40/P90或P1和P40/P90的突变体进行VIP分析，将在区分基因型中重要的某些拉曼谱带与特定的支原体表面蛋白组成和呈现相关联。变量选择及其与特定支原体表面成分的相关性，是开发该检测和基因分型平台的重要下一步。
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