Murugaiyan Jayaseelan, Eravci Murat, Weise Christoph, Roesler Uwe
Institute of Animal Hygiene and Environmental Health, Centre for Infectious Medicine, Freie Universität Berlin, Robert-von-Ostertag-Str. 7-13, 14163 Berlin, Germany.
Institute for Chemistry and Biochemistry, Freie Universität Berlin, Thielallee 63, 14195 Berlin, Germany.
Int J Mol Sci. 2016 Dec 29;18(1):59. doi: 10.3390/ijms18010059.
Microalgae of the genus () spp are associated with rare algal infections of invertebrates termed protothecosis. Among the seven generally accepted species, genotype 2 (GT2) is associated with a severe form of bovine mastitis while causes the mild and sub-clinical form of mastitis. The reason behind the infectious nature of GT2, while genotype 1 (GT1) remains non-infectious, is not known. Therefore, in the present study we investigated the protein expression level difference between the genotypes of and Cells were cultured to the mid-exponential phase, harvested, and processed for LC-MS analysis. Peptide data was acquired on an LTQ Orbitrap Velos, raw spectra were quantitatively analyzed with MaxQuant software and matching with the reference database of and resulted in the identification of 226 proteins. Comparison of an environmental strain with infectious strains resulted in the identification of 51 differentially expressed proteins related to carbohydrate metabolism, energy production and protein translation. The expression level of Hsp70 proteins and their role in the infectious process is worth further investigation. All mass spectrometry data are available via ProteomeXchange with identifier PXD005305.
（）属的微藻与无脊椎动物罕见的藻类感染（称为原藻病）有关。在七个普遍认可的物种中，基因型2（GT2）与严重形式的牛乳腺炎有关，而导致轻度和亚临床形式的乳腺炎。GT2具有传染性，而基因型1（GT1）仍无传染性，其背后的原因尚不清楚。因此，在本研究中，我们调查了和基因型之间的蛋白质表达水平差异。将细胞培养至指数中期，收获并进行LC-MS分析。在LTQ Orbitrap Velos上获取肽数据，用MaxQuant软件对原始光谱进行定量分析，并与和的参考数据库匹配，鉴定出226种蛋白质。将环境菌株与感染性菌株进行比较，鉴定出51种与碳水化合物代谢、能量产生和蛋白质翻译相关的差异表达蛋白质。Hsp70蛋白的表达水平及其在感染过程中的作用值得进一步研究。所有质谱数据可通过ProteomeXchange获得，标识符为PXD005305。
