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长链非编码RNA PCAT-1通过抑制人卵巢癌中的miR-129-5p促进肿瘤进展。

Long non-coding RNA PCAT-1 promotes tumor progression by inhibiting miR-129-5p in human ovarian cancer.

作者信息

Gu Li-Ping, Jin Shuo, Xu Rong-Chun, Zhang Jing, Geng Ying-Chun, Shao Xing-Yue, Qin Li-Bo

机构信息

Department of Obstetrics-Gynecology, Daqing Oil Field General Hospital, Daqing, Heilongjing, China.

出版信息

Arch Med Sci. 2019 Mar;15(2):513-521. doi: 10.5114/aoms.2018.75534. Epub 2018 Apr 30.

DOI:10.5114/aoms.2018.75534
PMID:30899305
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6425202/
Abstract

INTRODUCTION

Ovarian cancer (OC) is one of the most common malignancies and the leading cause of cancer-related death among women. The long non-coding RNA Prostate cancer-associated transcript-1 (PCAT-1) has been reported to play important roles in multiple human cancers. However, the role of PCAT-1 in OC has never been investigated. The purpose of this study was to investigate the expression and roles of PCAT-1 in OC.

MATERIAL AND METHODS

Expression of PCAT-1 and miR-129-5p in OC tissues and cell lines was determined by qRT-PCR. Cell proliferation and apoptosis were analyzed by MTT assay and flow cytometry, respectively. The interaction between PCAT-1 and miR-129-5p was demonstrated by luciferase reporter assay.

RESULTS

PCAT-1 is significantly upregulated in OC tissues and cell lines ( < 0.05). Overexpression of PCAT-1 promotes proliferation of OC cells and inhibits their apoptosis ( < 0.05). In addition, miR-129-5p is markedly downregulated in OC and its level is inversely correlated with PCAT-1 expression in OC tumor tissues ( < 0.05). miR-129-5p inhibits proliferation and induces apoptosis in OC cell lines ( < 0.05). Furthermore, it has been demonstrated that miR-129-5p is directly targeted by PCAT-1 and miR-129-5p overexpression can effectively attenuate the effects of PCAT-1 on the proliferation and apoptosis of OC cells.

CONCLUSIONS

Our results suggest that PCAT-1 functions as an oncogene by inhibiting miR-129-5p in OC and silencing PCAT-1 may be a novel therapeutic strategy in the treatment of OC.

摘要

引言

卵巢癌（OC）是最常见的恶性肿瘤之一，也是女性癌症相关死亡的主要原因。据报道，长链非编码RNA前列腺癌相关转录本1（PCAT-1）在多种人类癌症中发挥重要作用。然而，PCAT-1在OC中的作用尚未得到研究。本研究的目的是探讨PCAT-1在OC中的表达及作用。

材料与方法

采用qRT-PCR检测OC组织和细胞系中PCAT-1和miR-129-5p的表达。分别通过MTT法和流式细胞术分析细胞增殖和凋亡情况。通过荧光素酶报告基因实验证明PCAT-1与miR-129-5p之间的相互作用。

结果

PCAT-1在OC组织和细胞系中显著上调（<0.05）。PCAT-1的过表达促进OC细胞增殖并抑制其凋亡（<0.05）。此外，miR-129-5p在OC中明显下调，其水平与OC肿瘤组织中PCAT-1的表达呈负相关（<0.05）。miR-129-5p抑制OC细胞系的增殖并诱导其凋亡（<0.05）。此外，已证明miR-129-5p是PCAT-1的直接靶点，miR-129-5p的过表达可有效减弱PCAT-1对OC细胞增殖和凋亡的影响。

结论

我们的结果表明，PCAT-1在OC中通过抑制miR-129-5p发挥癌基因作用，沉默PCAT-1可能是治疗OC的一种新的治疗策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f24/6425202/915091994623/AMS-15-32714-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f24/6425202/31ebd38247ef/AMS-15-32714-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f24/6425202/c3c35b1b433a/AMS-15-32714-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f24/6425202/34980ea2e500/AMS-15-32714-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f24/6425202/915091994623/AMS-15-32714-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f24/6425202/31ebd38247ef/AMS-15-32714-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f24/6425202/c3c35b1b433a/AMS-15-32714-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f24/6425202/34980ea2e500/AMS-15-32714-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f24/6425202/915091994623/AMS-15-32714-g004.jpg

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