Chuang Augustine H, Bordlemay Justin, Goodin Jeremy L, McPherson James C
Department of Clinical Investigation, Dwight D. Eisenhower Army Medical Center, 7 Ave. Bldg 38705, Fort Gordon, GA.
Mil Med. 2019 Mar 1;184(Suppl 1):97-101. doi: 10.1093/milmed/usy332.
Sodium lauryl sulfate (SLS) is a surfactant used to decrease the surface tension of water. Most commercially available dentifrices contain 0.5-2.0% SLS. This study investigated the potential effect of SLS on oral wound healing using primary human gingival fibroblasts (HGFs).
HGFs cells were grown in12-well culture plates in DMEM medium. A 3 mm wound was created on confluent HGFs. The cells were challenged with 0 (the control group), 0.01, 0.02, 0.03, 0.04, or 0.05% SLS-containing media once daily for 2 minutes. The cells were stained on day 0, 2, 4, 6 and 8. The percent of wound fill area was measured.
On day 2, 4, 6, and 8, the wound fill of the control group (0% SLS) was 15, 35, 67 and 98%, respectively; at 0.01% SLS, it was 10, 20, 65 and 84%; at 0.02%, it was 7, 10, 15 and 25%; at 0.03% SLS, it was only 5% and 8% on day 2 and 4.
Our results showed a dose- and time-dependent inhibition on HGFs wound fill by SLS; however, future in vivo studies are needed to validate if our in vitro findings using SLS-free dentifrices to avoid the potential delay of wound healing.
月桂醇硫酸酯钠（SLS）是一种用于降低水表面张力的表面活性剂。大多数市售牙膏含有0.5 - 2.0%的SLS。本研究使用原代人牙龈成纤维细胞（HGFs）调查了SLS对口腔伤口愈合的潜在影响。
HGFs细胞在12孔培养板的DMEM培养基中生长。在汇合的HGFs上制造一个3毫米的伤口。细胞每天用含0（对照组）、0.01、0.02、0.03、0.04或0.05% SLS的培养基刺激2分钟。在第0、2、4、6和8天对细胞进行染色。测量伤口填充面积的百分比。
在第2、4、6和8天，对照组（0% SLS）的伤口填充率分别为15%、35%、67%和98%；0.01% SLS时，分别为10%、20%、65%和84%；0.02%时，分别为7%、10%、15%和25%；0.03% SLS时，在第2天和第4天仅为5%和8%。
我们的数据显示SLS对HGFs伤口填充有剂量和时间依赖性抑制作用；然而，需要未来的体内研究来验证我们使用不含SLS的牙膏的体外研究结果，以避免伤口愈合的潜在延迟。
