Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Chemistry, Payame Noor University, 19395-4697 Tehran, Iran; Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Food Chem. 2019 Aug 1;288:29-38. doi: 10.1016/j.foodchem.2019.02.087. Epub 2019 Feb 26.
In this study, core-shell lincomycin-imprinted polymers were successfully synthesized and their binding properties evaluated. The functional monomers of methacrylamide and acrylamide were used for synthesis of core and shell, respectively. The optimum synthesized core-shell molecularly imprinted polymer (MIP) was applied as a sorbet in solid phase extraction cartridge. Afterwards, the method of core-shell molecularly imprinted solid phase extraction (CSMISPE) was used for pre-concentration and clean-up of lincomycin in the milk matrix prior to analysis via high performance liquid chromatography equipped with UV detector (HPLC-UV). The linear range for analysis of lincomycin in the milk matrix using introduced method was obtained from 0.08 to 2 μg/mL with recovery range of 80%-89%. The limit of detection and limit of quantification were 0.02 μg/mL and 0.08 μg/mL, respectively. Finally, calibrated CSMISPE-HPLC-UV method was used for lincomycin residue checking and quantification in the pasteurized milk samples of Mashhad city market.
在这项研究中，成功合成了核壳型林可霉素印迹聚合物，并对其结合性能进行了评价。采用丙烯酰胺和甲基丙烯酰胺作为功能单体分别合成核和壳。最优合成的核壳型分子印迹聚合物（MIP）被用作固相萃取柱中的吸附剂。随后，采用核壳型分子印迹固相萃取（CSMISPE）法对牛奶基质中的林可霉素进行预浓缩和净化，然后通过高效液相色谱-紫外检测（HPLC-UV）进行分析。该方法在牛奶基质中分析林可霉素的线性范围为 0.08 至 2μg/mL，回收率范围为 80%至 89%。检测限和定量限分别为 0.02μg/mL 和 0.08μg/mL。最后，使用校准的 CSMISPE-HPLC-UV 法对马什哈德市市场的巴氏奶样品中的林可霉素残留量进行检测和定量。
