弧形支架诱导的膜形态由弧角和覆盖度决定。
Membrane Morphologies Induced by Arc-Shaped Scaffolds Are Determined by Arc Angle and Coverage.
机构信息
Max Planck Institute of Colloids and Interfaces, Department of Theory and Bio-Systems, Potsdam, Germany.
Max Planck Institute of Colloids and Interfaces, Department of Theory and Bio-Systems, Potsdam, Germany.
出版信息
Biophys J. 2019 Apr 2;116(7):1239-1247. doi: 10.1016/j.bpj.2019.02.017. Epub 2019 Feb 26.
The intricate shapes of biological membranes such as tubules and membrane stacks are induced by proteins. In this article, we systematically investigate the membrane shapes induced by arc-shaped scaffolds such as proteins and protein complexes with coarse-grained modeling and simulations. We find that arc-shaped scaffolds induce membrane tubules at membrane coverages larger than a threshold of ∼40%, irrespective of their arc angle. The membrane morphologies at intermediate coverages below this tubulation threshold, in contrast, strongly depend on the arc angle. Scaffolds with arc angles of about 60°, akin to N-BAR domains, do not change the membrane shape at coverages below the tubulation threshold, whereas scaffolds with arc angles larger than about 120° induce double-membrane stacks at intermediate coverages. The scaffolds stabilize the curved membrane edges that connect the membrane stacks, as suggested for complexes of reticulon proteins. Our results provide general insights on the determinants of membrane shaping by arc-shaped scaffolds.
生物膜的复杂形状，如管状和膜堆叠，是由蛋白质诱导的。在本文中，我们使用粗粒度建模和模拟系统地研究了由弧形支架（如蛋白质和蛋白质复合物）诱导的膜形状。我们发现，弧形支架在膜覆盖度大于约 40%时会诱导管状膜，而与弧形支架的弧形角度无关。相比之下，在这个管状化阈值以下的中间覆盖度下的膜形态强烈依赖于弧形角度。具有约 60°弧形角度的支架，类似于 N-BAR 结构域，在管状化阈值以下的覆盖度下不会改变膜形状，而具有大于约 120°弧形角度的支架在中间覆盖度下会诱导双层膜堆叠。支架稳定了连接膜堆叠的弯曲膜边缘，这与网蛋白复合物的复合物一致。我们的结果提供了关于弧形支架对膜成形决定因素的一般见解。
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