氧化锌纳米颗粒对尿路感染白色念珠菌菌株初始黏附及 ALS1 和 ALS3 基因表达的抑制作用。

Zinc Oxide Nanoparticles Inhibition of Initial Adhesion and ALS1 and ALS3 Gene Expression in Candida albicans Strains from Urinary Tract Infections.

机构信息

Laboratory Sciences Research center, Golestan University of Medical Sciences, Gorgan, Iran.

Department of Laboratory Sciences, Faculty of Paramedicine, Gorgan University of Medical Sciences, Gorgan, 4934174515, Iran.

出版信息

Mycopathologia. 2019 Apr;184(2):261-271. doi: 10.1007/s11046-019-00327-w. Epub 2019 Mar 22.

DOI:10.1007/s11046-019-00327-w

PMID:30903582

Abstract

OBJECTIVE

The aim of this study was to evaluate effects of zinc oxide nanoparticles (ZnO-np) on initiation adhesion and agglutinin-like sequence (ALS) 1 and ALS3 gene expression, which is the first cell surface protein known to be, required for biofilm formation in 125 Candida albicans (C. albicans) from urinary tract infections (UTIs).

METHODS

In this descriptive-analytic study, Candida UTIs was cultured from 280 women admitted in hospital Sayad Shirazi, in Northeastern Iran in the 2018 year. 125 (44.62%) of C. albicans strains were identified by phenotypic and genotypic methods. Susceptibility testing C. albicans strains were determined by the disk diffusion method. ZnO-np with 20-40 nm diameters were prepared, there were confirmed by the X-ray diffraction and scanning electron microscope methods.

RESULTS

115 susceptible C. albicans and 10 fluconazole-resistant C. albicans strain from UTIs were exposed to sub-minimum inhibitory concentration (Sub-MIC) of ZnO-np (range 0.02-18.1 μg/ml). Expression of the ALS1 and ALS3 genes which are affected by adhesion was evaluated by real-time PCR. One-way ANOVA test statistical analysis was performed with SPSS 16.0. The MIC range of ZnO-np was 0.05-296 µg/ml. Sub-MIC concentration ZnO-np initial adhesion inhibition, and significantly reduced ALS1 and ALS3 gene expression was observed in all strains (P < 0.05). The finding indicated that ZnO-np is effective in reduction of ALS1 and ALS3 expression in fluconazole-resistant strains.

CONCLUSIONS

Therefore, ZnO-np could be a candidate in the elimination of biofilm C. albicans strains from UTIs in medicine, particularly in medical instruments.

摘要

目的

本研究旨在评估氧化锌纳米粒子（ZnO-np）对引发黏附及凝集素样序列（ALS）1 和 ALS3 基因表达的影响，这是已知的第一个位于细胞表面的蛋白，对生物膜形成所必需的，在 125 株尿路白色念珠菌（C. albicans）中起作用。

方法

在这项描述性分析研究中，2018 年，从伊朗东北部 Sayad Shirazi 医院住院的 280 名女性中培养出了尿路白色念珠菌。通过表型和基因分型方法鉴定了 125 株（44.62%）C. albicans 株。用纸片扩散法测定念珠菌的药敏试验。用 X 射线衍射和扫描电子显微镜方法对 20-40nm 直径的 ZnO-np 进行了制备和确认。

结果

从 UTIs 中分离出 115 株敏感株和 10 株氟康唑耐药株 C. albicans，暴露于 ZnO-np 的亚最小抑菌浓度（Sub-MIC）（范围为 0.02-18.1μg/ml）。用实时 PCR 评估了黏附受影响的 ALS1 和 ALS3 基因的表达。用 SPSS 16.0 进行了单因素方差分析。ZnO-np 的 MIC 范围为 0.05-296μg/ml。在所有菌株中，Sub-MIC 浓度的 ZnO-np 对初始黏附有抑制作用，并且显著降低了 ALS1 和 ALS3 基因的表达（P<0.05）。结果表明，ZnO-np 能有效降低氟康唑耐药株中 ALS1 和 ALS3 的表达。