Hosseini Seyedeh Sedigheh, Yadegari Mohammad Hossein, Rajabibazl Masoumeh, Ghaemi Ezzat Allah
Department of Medical Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Iran J Microbiol. 2016 Dec;8(6):401-409.
Secreted Aspartyl Proteinase (SAP) is one of the main virulence factors in the pathogenesis of This enzyme is encoded by a family of at least ten genes. Among these genes, the role of in mucosal infections is evident. This study aimed to investigate the expression of genes of isolates after treatment with extract, carvacrol and caspofungin drug.
Vaginal samples of 68 women with suspected vaginitis were obtained and cultured. species were identified using phenotypic and genotyping methods. Spectrophotometry was used to investigate the presence of protein in the vaginal samples, and SDS-PAGE was used to confirm its protein composition. Real-time PCR was performed to ascertain the effects of subinhibitory concentrations of extract, carvacrol and caspofungin on the expression of genes before and after treatment.
was found as the abundant species (59.6%), and different amounts of were present in all vaginal samples, which were higher than strain. The protein composition of in samples was estimated with the approximate molecular weight of 45 kDa. levels of total in FLU-resistant isolates (P=0.01) were more than those of FLU-susceptible isolates (P=0.07). The findings indicated that carvacrol is effective in reduction of expression with a particular effect against FLU-resistant isolates.
Carvacrol contains an essential oil (carvacrol); therefore, it can be considered as an alternative effective antifungal compound.
分泌型天冬氨酸蛋白酶（SAP）是[疾病名称]发病机制中的主要毒力因子之一。该酶由至少十个基因组成的家族编码。在这些基因中，[具体基因名称]在黏膜感染中的作用较为明显。本研究旨在探讨用[提取物名称]提取物、香芹酚和卡泊芬净药物处理后[病原体名称]分离株的[基因名称]基因表达情况。
采集68例疑似阴道炎女性的阴道样本并进行培养。采用表型和基因分型方法鉴定[病原体名称]种类。用分光光度法检测阴道样本中[蛋白质名称]的存在情况，并用SDS - PAGE法确认其蛋白质组成。进行实时PCR以确定亚抑菌浓度的[提取物名称]提取物、香芹酚和卡泊芬净在处理前后对[基因名称]基因表达的影响。
发现[病原体名称]为优势菌种（59.6%），所有阴道样本中均存在不同量的[蛋白质名称]，且高于[菌株名称]菌株。[病原体名称]样本中[蛋白质名称]的蛋白质组成估计其近似分子量为45 kDa。耐氟康唑分离株中总[蛋白质名称]水平（P = 0.01）高于氟康唑敏感分离株（P = 0.07）。研究结果表明，香芹酚可有效降低[基因名称]的表达，对耐氟康唑分离株有特别的作用效果。
香芹酚含有一种精油（香芹酚）；因此，它可被视为一种有效的抗真菌替代化合物。
