克氏锥虫：前鞭毛体和锥鞭毛体的多肽标志物

Trypanosoma cruzi: polypeptide markers of epimastigotes and trypomastigotes.

作者信息

Rangel-Aldao R, Comach G, Allende O, Cayama E, Delgado V, Piras R, Piras M, Henriquez D, Negri S

出版信息

Mol Biochem Parasitol. 1986 Jul;20(1):25-32. doi: 10.1016/0166-6851(86)90139-8.

Abstract

We compared the major polypeptides of epimastigotes and trypomastigotes of T. cruzi, by submitting total parasite lysates to electrophoresis in polyacrylamide gels (SDS-PAGE), protein staining with Coomassie brilliant blue, laser densitometry, or immunoblotting with sera derived from infected individuals (Chagas' disease). Epimastigotes and trypomastigotes displayed extensive homology, the differences being quantitative, except for a trypomastigote-specific band of Mr 75,000 which reacted with chagasic sera. Immunoblotting with chagasic sera confirmed the electrophoretic homology of epimastigotes and trypomastigotes. Upon antigenic dilution, a cluster of antigenic bands in the range of Mr 150,000 to 75,000 prevailed in the trypomastigotes, whereas the epimastigotes displayed more abundance of antigenic bands in the range of Mr 72,000 to 36,000.

摘要

我们通过将总的寄生虫裂解物进行聚丙烯酰胺凝胶电泳（SDS-PAGE）、用考马斯亮蓝进行蛋白质染色、激光密度测定法，或用来自感染个体（恰加斯病）的血清进行免疫印迹，比较了克氏锥虫前鞭毛体和锥鞭毛体的主要多肽。前鞭毛体和锥鞭毛体显示出广泛的同源性，差异是定量性的，除了一条分子量为75,000的锥鞭毛体特异性条带，它能与恰加斯病血清发生反应。用恰加斯病血清进行免疫印迹证实了前鞭毛体和锥鞭毛体的电泳同源性。在抗原稀释后，分子量在150,000至75,000范围内的一组抗原条带在锥鞭毛体中占优势，而前鞭毛体在分子量72,000至36,000范围内显示出更多的抗原条带。