Ding Mingya, Bai Yun, Li Jin, Yang Xuejing, Wang Hui, Gao Xiumei, Chang Yan-Xu
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
Front Pharmacol. 2019 Mar 8;10:227. doi: 10.3389/fphar.2019.00227. eCollection 2019.
A simple and eco-friendly Diol-based-matrix solid-phase dispersion method (MSPD) was optimized and established to simultaneously extract 13 bioactive compounds (7 coumarins and 6 phenolic acids) in (APR) by ultrahigh performance liquid chromatography coupled with photodiode array detector (UHPLC-PDA). Diol was chosen as the dispersing sorbent and methanol solution was used as the elution solvent. The preparation procedures for the MSPD including the types of sorbents, mass ratio of matrix to sorbent, grinding time, type, concentration and volume of elution solvent were investigated. Under the optimal conditions, good recoveries of the 13 target compounds were obtained in the range of 94.8-107% (RSD < 3.22%). The limits of detection (LODs) and limits of quantitation (LOQs) were in the ranges of 0.08-0.12 μg mL and 0.16-0.24 μg mL, respectively. Compared with the traditional method, it was a green and environmentally friendly technique. The results proved that the established method was successfully applied to the extraction and determination of 13 target bioactive compounds for quality control in APR.
优化并建立了一种简单且环保的基于二醇基质的固相分散法（MSPD），用于通过超高效液相色谱-光电二极管阵列检测器（UHPLC-PDA）同时提取当归（APR）中的13种生物活性化合物（7种香豆素和6种酚酸）。选择二醇作为分散吸附剂，甲醇溶液作为洗脱溶剂。研究了MSPD的制备程序，包括吸附剂类型、基质与吸附剂的质量比、研磨时间、洗脱溶剂的类型、浓度和体积。在最佳条件下，13种目标化合物的回收率良好，在94.8%-107%范围内（相对标准偏差<3.22%）。检测限（LODs）和定量限（LOQs）分别在0.08-0.12μg/mL和0.16-0.24μg/mL范围内。与传统方法相比，这是一种绿色环保技术。结果证明，所建立的方法成功应用于当归质量控制中13种目标生物活性化合物的提取和测定。
