Mohamadi Fereshteh, Shakibapour Mahshid, Sharafi Seyedeh Maryam, Reza Ali Andalib, Tolouei Sepideh, Darani Hossein Yousofi
Department of Medical Parasitology and Mycology, Isfahan University of Medical Sciences, Isfahan 8179498861, Iran.
Environment Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan 8179498861, Iran.
Biomed Rep. 2019 Mar;10(3):183-188. doi: 10.3892/br.2019.1186. Epub 2019 Jan 17.
is prevalent intracellular parasite and a cause of worldwide infection in the human population. An inhibitory effect of this parasite on cancer growth has been demonstrated in cell culture and animal models. To determine whether the anticancer activities of are associated with host immune response, in the current study the reactivity of anti- antiserum with the surface of cancer cell lines was investigated. Anti- antibodies were raised in rabbit and the reaction of this antiserum in comparison with other anti-parasite antisera (anti-, anti-hydatid cyst fluid, anti-protoscolices antigens) with mouse melanoma or breast cancer cells lines was investigated using flow cytometry. Anti- antiserum reacted markedly with the surface of mouse melanoma and breast cancer cells, and less so with the normal mouse spleen lymphocytes. Meanwhile, the other anti-parasite antisera did not react strongly with the surface of cancer cells compared with normal mouse spleen lymphocytes. In summary, it has been demonstrated herein that anti- antiserum may selectively react with the surface of mouse cancer cells but not with normal mouse spleen lymphocytes. Therefore, further study on anti- antibodies may be useful for directing the application of selective drug delivery in cancer treatment.
是一种普遍存在的细胞内寄生虫，也是全球人类感染的一个病因。这种寄生虫对癌症生长的抑制作用已在细胞培养和动物模型中得到证实。为了确定其抗癌活性是否与宿主免疫反应相关，在本研究中，研究了抗血清与癌细胞系表面的反应性。在兔体内产生抗抗体，并使用流式细胞术研究该抗血清与其他抗寄生虫抗血清（抗、抗包虫囊肿液、抗原头节抗原）与小鼠黑色素瘤或乳腺癌细胞系的反应。抗血清与小鼠黑色素瘤和乳腺癌细胞表面有明显反应，而与正常小鼠脾淋巴细胞的反应较弱。同时，与正常小鼠脾淋巴细胞相比，其他抗寄生虫抗血清与癌细胞表面的反应不强。总之，本文已证明抗血清可能与小鼠癌细胞表面选择性反应，而不与正常小鼠脾淋巴细胞反应。因此，对抗抗体的进一步研究可能有助于指导选择性药物递送在癌症治疗中的应用。
