REQUIMTE/LAQV, Faculty of Pharmacy, University of Porto, Rua Jorge Viterbo Ferreira, 280, 4050-313 Porto, Portugal.
Food Funct. 2019 Apr 17;10(4):2050-2060. doi: 10.1039/c8fo02536c.
In the present study, a coffee-like beverage was prepared from Quercus cerris seeds. Seeds presented 60.4% carbohydrates (of which 26.9% is fiber), 6.3% proteins and 4.0% fat. In the coffee-like beverage, the TPC and TFC were 2070.2 mg GAE per L and 285.3 mg CEQ per L, respectively. The IC50 values determined using FRAP and DPPH assays were, respectively, 203.1 mg L-1 and 271.6 mg L-1. Concerning the reactive species scavenging capacity, the extract was more effective against O2˙-, HClO and ˙NO (namely, IC50 = 17.24 ± 0.24 μg mL-1, IC50 = 9.25 ± 0.92 μg mL-1 and IC50 = 0.65 ± 0.06 μg mL-1). Qualitative and quantitative analyses by 1D- and 2D-NMR and LC-MS indicated high levels of ellagic acid and gallotannin or ellagitannin derivatives. The optimal range of non-cytotoxic concentrations in Caco-2 and HT29-MTX cell lines was between 0.1 and 1.0 μg mL-1. This is the first study that evaluates the Q. cerris seed extract as a beverage.
在本研究中，从栓皮栎种子中制备出一种类似咖啡的饮料。种子含有 60.4%的碳水化合物（其中 26.9%为纤维）、6.3%的蛋白质和 4.0%的脂肪。在这种类似咖啡的饮料中，TPC 和 TFC 分别为 2070.2 mg GAE/L 和 285.3 mg CEQ/L。使用 FRAP 和 DPPH 测定法确定的 IC50 值分别为 203.1 mg L-1和 271.6 mg L-1。就活性物质清除能力而言，该提取物对 O2˙-、HClO 和 ˙NO 的清除效果更为显著（即，IC50=17.24±0.24μg/mL、IC50=9.25±0.92μg/mL 和 IC50=0.65±0.06μg/mL）。通过 1D-和 2D-NMR 和 LC-MS 的定性和定量分析表明，鞣花酸和没食子单宁或鞣花单宁衍生物的含量很高。在 Caco-2 和 HT29-MTX 细胞系中，非细胞毒性浓度的最佳范围在 0.1 到 1.0 μg/mL 之间。这是首次评估栓皮栎种子提取物作为饮料的研究。
