Department of Plant, Food, and Environmental Sciences, Dalhousie University, Truro, NS, Canada.
PLoS One. 2019 Mar 25;14(3):e0214376. doi: 10.1371/journal.pone.0214376. eCollection 2019.
Little is known about the ecology of microbial plastic degradation. In this study, we employed next generation amplicon sequencing to assess the effect of low-density polyethylene (LDPE) films on the structure of bacterial and fungal communities in four mature compost piles with age ranging between 2 and 10 years. While, bacterial Proteobacteria, Bacteroidetes, Actinobacteria and fungi Ascomycota were most abundant across all facilities, our data indicated significant differences in compost microbiomes between compost facilities, which might be related to compost chemical parameters, age of piles and characteristics of the feedstock. In addition, a substantial shift in the interaction pattern within microbial communities from bulk and plastic-associated (PA) compost was detected. For example, cooperation between Firmicutes Bacillaceae and Thermoactinomycetaceae was detected only in PA compost. However, based on the analysis of the diversity indices and the relative abundances of microbial taxa we can conclude that the presence of plastics in compost had no significant effect on the structure of microbial community.
关于微生物塑料降解的生态学知之甚少。在这项研究中，我们采用下一代扩增子测序来评估低密度聚乙烯 (LDPE) 薄膜对四个成熟堆肥中细菌和真菌群落结构的影响，这些堆肥的年龄在 2 到 10 年之间。尽管细菌门的 Proteobacteria、Bacteroidetes、Actinobacteria 和真菌门的 Ascomycota 在所有设施中都最为丰富，但我们的数据表明堆肥设施之间的堆肥微生物群落存在显著差异，这可能与堆肥的化学参数、堆肥的年龄和原料的特性有关。此外，还检测到从堆肥本体和与塑料相关（PA）堆肥中微生物群落内部相互作用模式的重大转变。例如，Firmicutes 芽孢杆菌科和 Thermoactinomycetaceae 之间的合作仅在 PA 堆肥中检测到。然而，根据多样性指数和微生物分类群相对丰度的分析，我们可以得出结论，塑料在堆肥中的存在对微生物群落的结构没有显著影响。
