Dibb W L, Digranes A, Bottolfsen K L
Acta Pathol Microbiol Immunol Scand B. 1986 Jun;94(3):173-6. doi: 10.1111/j.1699-0463.1986.tb03038.x.
The in vitro activity of erythromycin against clinical isolates of Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, Streptococcus pyogenes and Haemophilus influenzae was examined by agar dilution and agar diffusion methods. The plates were incubated in air alone or in 8% CO2 and air. The minimal inhibitory concentrations (MICs) measured in air alone were lower for most of the isolates, compared to those found in 8% CO2. The greatest differences in MIC values were found for H. influenzae; the MIC 50% was 0.5 mg/l in air and 4 mg/l in 8% CO2. Sensitivity testing by the agar diffusion method (ICS) showed considerable differences between results obtained in air and in 8% CO2; the inhibition zones were generally smaller in CO2. The most marked reduction in zone sizes after incubation in 8% CO2 was seen with the H. influenzae isolates; 15 out of 43 isolates moved from the "sensitive" to "moderately sensitive" group. Sensitivity determination of aerobic bacteria for erythromycin should be performed in air alone in the routine laboratory.
采用琼脂稀释法和琼脂扩散法检测了红霉素对金黄色葡萄球菌、表皮葡萄球菌、肺炎链球菌、化脓性链球菌和流感嗜血杆菌临床分离株的体外活性。平板分别在纯空气中或8%二氧化碳与空气混合环境中孵育。与在8%二氧化碳环境中相比，多数分离株在纯空气中测得的最低抑菌浓度（MIC）较低。流感嗜血杆菌的MIC值差异最大；50% MIC在空气中为0.5mg/l，在8%二氧化碳中为4mg/l。琼脂扩散法（ICS）敏感性试验显示，在空气和8%二氧化碳环境中获得的结果存在显著差异；在二氧化碳环境中抑菌圈通常较小。流感嗜血杆菌分离株在8%二氧化碳中孵育后抑菌圈大小减小最为明显；43株分离株中有15株从“敏感”组变为“中度敏感”组。常规实验室中，需在纯空气中对需氧菌进行红霉素敏感性测定。
