Department of Biology, Faculty of Science, Taif University, Taif 11099, Saudi Arabia.
Unité de Recherche: Virologie & stratégies antivirales, Institut Supérieur de Biotechnologie, Monastir University, Monastir 5000, Tunisia.
Molecules. 2019 Mar 23;24(6):1161. doi: 10.3390/molecules24061161.
Urinary tract infections (UTIs), caused by 80% to 85% of the time, are one of the most important causes of morbidity and health care spending affecting persons of all ages. These infections lead to many difficult problems, especially increasing resistance to antibiotic drugs. Bacterial biofilms play an important role in UTIs, responsible for persistent infections leading to recurrences and relapses. In this study, we have investigated the antibacterial activity of five medicinal plant essential oils against UTIs caused by using disc diffusion and minimal inhibition concentration (MIC) methods. In addition, biofilm inhibitory action of oils was realized by crystal violet. Gas chromatography⁻mass spectrometry (GC⁻MS) analysis showed a variability between oils in terms of compound numbers as well as their percentages. Antibacterial activity was observed only in cases of , and , while and did not showed any effect towards isolates. essential oil demonstrated the highest antibacterial activity against isolates, followed by and . Further, oils showed high biofilm inhibitory action with a percentage of inhibition that ranged from 14.94% to 94.75%. oil had the highest antibiofilm activity followed by and . Accordingly, tested oils showed very effective antibacterial and antibiofilm activities against UTIs and can be considered as good alternative for antibiotics substitution.
尿路感染（UTIs），80%到 85%的情况下是由引起的，是影响所有年龄段人群发病率和医疗保健支出的最重要原因之一。这些感染导致了许多困难的问题，特别是对抗生素药物的耐药性增加。细菌生物膜在 UTIs 中起着重要作用，导致持续感染，从而导致复发和反复感染。在这项研究中，我们使用圆盘扩散和最小抑菌浓度（MIC）方法研究了五种药用植物精油对 UTIs 的抗菌活性。此外，通过结晶紫法实现了油对生物膜的抑制作用。气相色谱⁻质谱（GC⁻MS）分析表明，在化合物数量及其百分比方面，油之间存在可变性。仅在 、 和 情况下观察到抗菌活性，而 和 对 分离株没有任何作用。 精油对 分离株表现出最高的抗菌活性，其次是 和 。此外，这些油对生物膜表现出很高的抑制作用，抑制率从 14.94%到 94.75%不等。 油具有最高的抗生物膜活性，其次是 和 。因此，测试的油对 UTIs 表现出非常有效的抗菌和抗生物膜活性，可以被认为是抗生素替代物的良好选择。
