Ksouri S, Djebir S, Bentorki A A, Gouri A, Hadef Y, Benakhla A
Department of Biology, Faculty of Nature and Life Sciences and Earth Sciences and the Universe, University of 8 Mai 1945 Guelma, BP 401, 24000 Guelma, Algeria.
Department of Biology, Faculty of Nature and Life Sciences and Earth Sciences and the Universe, University of 8 Mai 1945 Guelma, BP 401, 24000 Guelma, Algeria.
J Mycol Med. 2017 Jun;27(2):245-249. doi: 10.1016/j.mycmed.2017.03.004. Epub 2017 Apr 25.
The aim of this study is to limit the antibiotic use in mastitis treatment and to find other alternatives. The antifungal activity of the essential oils from Origanum floribundum Munby., Rosmarinus officinalis L. and Thymus ciliatus Desf. is studied in the present work against a Candida albicans reference strain and ten C. albicans isolated strains from bovine clinical mastitis.
Essential oils were extracted by hydrodistillation technique using Clevenger apparatus. Their chromatographic analysis was performed with a Gas Chromatograph/Mass Spectrometer (GC/MS). Antifungal activities of essential oils were investigated by macrobroth method of dilution in tubes to determine the Minimum Inhibitory Concentrations (MIC 80%).
Analysis of the essential oil showed chemical profile dominated by thymol (50.47 and 62.41%) and P-cymene (24.22 and 15.51%) in the oregano and the thyme respectively, 1, 8-cineol (31.50%) and α-pinene (18.33%) in Rosemary. The three essential oils revealed highly effective anticandidal activity, with an MIC of 80% values ranged from 15.02 to 31.08μg/mL.
These results suggest that essential oils studied can be real alternatives in the control of mastitis fungi but deserving studies more in-depth and detailed on their application in vivo.
本研究旨在限制乳腺炎治疗中抗生素的使用并寻找其他替代方法。本文研究了多花牛至（Origanum floribundum Munby.）、迷迭香（Rosmarinus officinalis L.）和睫毛百里香（Thymus ciliatus Desf.）精油对白色念珠菌参考菌株以及从牛临床乳腺炎分离出的10株白色念珠菌菌株的抗真菌活性。
使用克莱文杰装置通过水蒸馏技术提取精油。用气相色谱/质谱仪（GC/MS）进行色谱分析。通过试管中的大肉汤稀释法研究精油的抗真菌活性，以确定最低抑菌浓度（MIC 80%）。
精油分析表明，牛至精油中百里香酚（分别为50.47%和62.41%）和对伞花烃（分别为24.22%和15.51%）占主导，迷迭香精油中1,8-桉叶素（31.50%）和α-蒎烯（18.33%）占主导，百里香精油中百里香酚和对伞花烃占主导。这三种精油均显示出高效的抗念珠菌活性，MIC 80%值范围为15.02至31.08μg/mL。
这些结果表明，所研究的精油可成为控制乳腺炎真菌的真正替代方法，但在其体内应用方面值得进行更深入和详细的研究。
