Reza Malekpour Afshar, Reza Mollaie Hamid, Mahdiyeh Lashkarizadeh, Mehdi Fazlalipour, Hamid Zeinali Nejad
Kerman University of Medical sciences, Afzalipour School of Medicine, Research Center of Infectious and Tropical Diseases, Kerman, Iran E-mail :
Asian Pac J Cancer Prev. 2015;16(16):7351-7. doi: 10.7314/apjcp.2015.16.16.7351.
Breast cancer is the most common cancer among women worldwide. Roles of the Epstein-Barr, Merkel cell polyoma and mouse mammary tumor viruses in breast carcinogenesis are still controversial although any relationship would clearly be important for breast cancer etiology, early detection and prevention. In the present study associations between EBV, MMTV and Merkel cell polyoma virus and breast cancer in 100 Iranian patients were evaluated using paraffin-embedded tissues. EBER RNA and expression of p53 and large T antigen were evaluated by real time PCR and CD34, p63, HER2, PR and ER markers were studied by immunohistochemistry. EBV was detected in 8/100 (8%), MMTV in 12/100 (12%), MPy in 3/100 (3%) and EBER RNA in 18/100 (18%) cases. None of the control samples demonstrated any of the viruses. p53 was suppressed in EBV, MPy and MMTV positive samples. The large T antigen rate was raised in MPy positive samples. Our results showed that EBV, MMTV and the Merkel cell polyoma virus are foundwith some proportion of breast cancers in our patients, suggesting that these viruses might have a significant role in breast cancer in Kerman, southeast of Iran.
乳腺癌是全球女性中最常见的癌症。尽管爱泼斯坦-巴尔病毒（EBV）、默克尔细胞多瘤病毒和小鼠乳腺肿瘤病毒在乳腺癌发生中的作用仍存在争议，但它们之间的任何关联显然对乳腺癌的病因、早期检测和预防都很重要。在本研究中，使用石蜡包埋组织评估了100名伊朗患者中EBV、MMTV和默克尔细胞多瘤病毒与乳腺癌之间的关联。通过实时PCR评估EBER RNA以及p53和大T抗原的表达，并通过免疫组织化学研究CD34、p63、HER2、PR和ER标志物。在100例患者中有8例（8%）检测到EBV，12例（12%）检测到MMTV，3例（3%）检测到MPy，18例（18%）检测到EBER RNA。对照样本均未显示任何病毒。在EBV、MPy和MMTV阳性样本中p53受到抑制。在MPy阳性样本中大T抗原率升高。我们的结果表明，在我们的患者中，EBV、MMTV和默克尔细胞多瘤病毒在一定比例的乳腺癌中被发现，这表明这些病毒可能在伊朗东南部克尔曼的乳腺癌中起重要作用。
