多头绒泡菌中的DNA复制：成熟的5-溴脱氧尿苷取代DNA的紫外光解
DNA replication in Physarum polycephalum: UV photolysis of maturing 5-bromo-deoxyuridine substituted DNA.
作者信息
Funderud S, Andreassen R, Haugli F
出版信息
Nucleic Acids Res. 1978 Sep;5(9):3303-13. doi: 10.1093/nar/5.9.3303.
Abstract
Combinations of 5-bromodeoxyuridine (BrdUrd) and 3H-deoxyadenosine (3H-DAdo) short pulses were given in the synchronous DNA-replication period of Physarum polycephalum. After a chase period, UV-photolysis products were analyzed on alkaline sucrose gradients. This strategy has allowed the following conclusions. a) at the time of master-initiation of DNA replication, points separated by 1.1-2.2x10(7) daltons of single strand DNA may initiate DNA synthesis. b) among these, only selected groups of replicons actually proceed in DNA replication at this time, while others appear to hold (later temporal sets of replicons). The origins of the ones that proceed in replication are separated from each other by a distance corresponding to 1.1-2.x10(7) daltons. c) regions in actual replication are separated from each other by increasing distances (up to 1.5x10(8) daltons single strand DNA) at later times in S.
摘要
在多头绒泡菌的同步DNA复制期，给予5-溴脱氧尿苷（BrdUrd）和3H-脱氧腺苷（3H-DAdo）短脉冲的组合。经过追踪期后，在碱性蔗糖梯度上分析紫外线光解产物。该策略得出了以下结论。a）在DNA复制的主起始时，由1.1 - 2.2×10⁷道尔顿单链DNA分隔的位点可能起始DNA合成。b）其中，此时只有选定的复制子组实际进行DNA复制，而其他复制子似乎处于停滞状态（后期的复制子时间组）。进行复制的复制子的起始点彼此之间的距离对应于1.1 - 2×10⁷道尔顿。c）在S期的后期，实际复制区域之间的距离逐渐增加（单链DNA可达1.5×10⁸道尔顿）。
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