多头绒泡菌中的DNA复制：复制子内DNA的双向复制。
DNA replication in Physarum polycephalum: bidirectional replication of DNA within replicons.
作者信息
Funderud S, Andreassen R, Haugli F
出版信息
Nucleic Acids Res. 1978 Mar;5(3):713-21. doi: 10.1093/nar/5.3.713.
Abstract
The direction of replication of DNA within replicons of Physarum polycephalum was studied by pulse-labelling with 5-bromouracil-deoxyriboside (BrdUrd) and 3H-adenosine deoxyriboside (dAdo), followed by ultraviolet- (UV) -photolysis and analysis of molecular weights of single strand DNA fragments on alkaline sucrose gradients. Newly made DNA within replicons at all stages of completion is split in two equal halves upon UV irradiation when BrdUrd was given at the time of initiation of DNA synthesis. This shows that replication within replicons of Physarum polycephalum starts at an origin located in the center of each unit, proceeding bidirectionally from this origin.
摘要
通过用5-溴尿嘧啶脱氧核糖核苷（BrdUrd）和3H-腺苷脱氧核糖核苷（dAdo）进行脉冲标记，随后进行紫外线（UV）光解并在碱性蔗糖梯度上分析单链DNA片段的分子量，研究了多头绒泡菌复制子内DNA的复制方向。当在DNA合成起始时给予BrdUrd时，处于完成各阶段的复制子内新合成的DNA在紫外线照射下会被分成两个相等的部分。这表明多头绒泡菌复制子内的复制从位于每个单元中心的一个起点开始，从该起点双向进行。
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