多头绒泡菌中的DNA复制：体内复制产物的特征
DNA replication in Physarum polycephalum: characterization of replication products in vivo.
作者信息
Funderud S, Haugli F
出版信息
Nucleic Acids Res. 1975 Aug;2(8):1381-90. doi: 10.1093/nar/2.8.1381.
Abstract
Synchronous plasmodia of Physarum polycephalum in DNA synthesis were pulse-labelled with [oH]- thymidine for time periods of 15 seconds up to 9 minutes, or given a 30 seconds pulse followed by chase periods of 9 minutes up to 6 hours. Sedimentation analysis in alkaline sucrose gradients revealed at least five species of single stranded DNA14 molecules in the pulse experiments. Co-sedimentation of [14C]-labelled phage-DNA gave relative S-values of 5-7, 13-15, 23-25, 30 and 33-35 for these DNA molecules, all of which can be chased into DNA of higher molecular weight.
摘要
多头绒泡菌同步化的原质团在DNA合成期用[³H] - 胸腺嘧啶核苷进行脉冲标记，标记时间从15秒至9分钟不等，或者进行30秒的脉冲标记，随后追踪9分钟至6小时。碱性蔗糖梯度沉降分析显示，在脉冲实验中至少有五种单链DNA¹⁴分子。[¹⁴C]标记的噬菌体DNA的共沉降分析得出这些DNA分子的相对S值分别为5 - 7、13 - 15、23 - 25、30和33 - 35，所有这些分子都能追踪到更高分子量的DNA。
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