Department of Implantology, Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou, People's Republic of China.
Department of Science and Education, Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou, People's Republic of China.
J Biomed Mater Res B Appl Biomater. 2020 Jan;108(1):80-93. doi: 10.1002/jbm.b.34368. Epub 2019 Mar 25.
Commercial bone substitute, such as calcined bovine bone (CBB), is currently extensively used as an alternative to autogenous bone. However, CBB lacks osteoinductivity and merely serves as a scaffold for native bone formation. To address this issue, we designed and prepared a heptaglutamate (E7)-modified BMP2-mimicking peptide (7E) and carried out a series of comprehensive physical characterizations and in vivo and in vitro studies to evaluate its role in the repair of cranial defects. The data elucidated that the amount of peptide anchoring to the bone graft materials was remarkably increased after modified with E7. Of note, 7E had a relatively stable and durable release, which promoted the osteogenic differentiation of rat derived bone marrow mesenchymal stem cells (BMSCs) and enhanced the bone regeneration of a rabbit calvarial defect by regulating the expression of the Runx2/SP7 axis. In summary, the composite biomaterials incorporating the E7-modified BMP2-mimicking peptide and CBB prepared in this study is a novel bone augmentation material with the merits of non-immunotoxicity, convenience, and low cost. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:80-93, 2020.
商业性骨替代物，如煅烧牛骨（CBB），目前被广泛用作自体骨的替代品。然而，CBB 缺乏成骨诱导性，只能作为天然骨形成的支架。为了解决这个问题，我们设计并制备了七谷氨酸（E7）修饰的 BMP2 模拟肽（7E），并进行了一系列全面的物理特性和体内、体外研究，以评估其在颅缺损修复中的作用。数据表明，E7 修饰后，肽与骨移植材料的结合量显著增加。值得注意的是，7E 具有相对稳定和持久的释放，通过调节 Runx2/SP7 轴的表达，促进大鼠骨髓间充质干细胞（BMSCs）的成骨分化，并增强兔颅骨缺损的骨再生。综上所述，本研究制备的 E7 修饰 BMP2 模拟肽与 CBB 复合的生物材料是一种新型的骨增强材料，具有非免疫毒性、方便和低成本的优点。© 2019 Wiley Periodicals, Inc. J 生物材料研究杂志 B：应用生物材料 108B:80-93, 2020.
