Morphology Group, Biomedical Research Institute, Hasselt University, Diepenbeek, Belgium.
Belgian Nuclear Research Centre, Radiobiology Unit, SCK•CEN, Mol, Belgium.
Dentomaxillofac Radiol. 2019 Sep;48(6):20180428. doi: 10.1259/dmfr.20180428. Epub 2019 Apr 5.
Cone-beam CT (CBCT) is a medical imaging technique used in dental medicine. However, there are no conclusive data available indicating that exposure to X-ray doses used by CBCT are harmless. We aim, for the first time, to characterize the potential age-dependent cellular and subcellular effects related to exposure to CBCT imaging. Current objective is to describe and validate the protocol for characterization of cellular and subcellular changes after diagnostic CBCT.
Development and validation of a dedicated two-part protocol: 1) assessing DNA double strand breaks (DSBs) in buccal mucosal (BM) cells and 2) oxidative stress measurements in saliva samples. BM cells and saliva samples are collected prior to and 0.5 h after CBCT examination. BM cells are also collected 24 h after CBCT examination. DNA DSBs are monitored in BM cells via immunocytochemical staining for γH2AX and 53BP1. 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dG) and total antioxidant capacity are measured in saliva to assess oxidative damage.
Validation experiments show that sufficient BM cells are collected (97.1 ± 1.4 %) and that γH2AX/53BP1 foci can be detected before and after CBCT examination. Collection and analysis of saliva samples, either sham exposed or exposed to IR, show that changes in 8-oxo-dG and total antioxidant capacity can be detected in saliva samples after CBCT examination.
The DIMITRA Research Group presents a two-part protocol to analyze potential age-related biological differences following CBCT examinations. This protocol was validated for collecting BM cells and saliva and for analyzing these samples for DNA DSBs and oxidative stress markers, respectively.
锥形束 CT（CBCT）是一种应用于牙科医学的医学成像技术。然而，目前尚无确凿的数据表明 CBCT 所使用的 X 射线剂量是无害的。我们首次旨在描述和验证用于描述与 CBCT 成像相关的潜在年龄相关细胞和亚细胞变化的方案。目前的目标是描述和验证用于描述与 CBCT 成像相关的潜在年龄相关细胞和亚细胞变化的方案。
开发和验证一个专用的两部分方案：1）评估颊黏膜（BM）细胞中的 DNA 双链断裂（DSB），2）评估唾液样本中的氧化应激测量。在 CBCT 检查前和检查后 0.5 小时采集 BM 细胞和唾液样本。在 CBCT 检查后 24 小时还采集 BM 细胞。通过免疫细胞化学染色检测 BM 细胞中的 γH2AX 和 53BP1 来监测 DNA DSB。通过测量唾液中的 8-氧代-7,8-二氢-2'-脱氧鸟苷（8-氧代-dG）和总抗氧化能力来评估氧化损伤。
验证实验表明，可以收集到足够的 BM 细胞（97.1±1.4%），并且可以在 CBCT 检查前后检测到 γH2AX/53BP1 焦点。收集和分析 sham 暴露或暴露于 IR 的唾液样本表明，在 CBCT 检查后可以在唾液样本中检测到 8-氧代-dG 和总抗氧化能力的变化。
DIMITRA 研究小组提出了一个两部分方案，用于分析 CBCT 检查后潜在的与年龄相关的生物学差异。该方案已通过收集 BM 细胞和唾液以及分析这些样本中的 DNA DSB 和氧化应激标志物进行了验证。
