Department of Oral and Maxillofacial Radiology, Faculty of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Mutat Res Genet Toxicol Environ Mutagen. 2022 Jul-Aug;879-880:503496. doi: 10.1016/j.mrgentox.2022.503496. Epub 2022 May 7.
This study sought to assess the cytotoxicity, genotoxicity, and immunohistochemical (IHC) expression of p53 in the oral mucosal epithelium of adults following cone-beam computed tomography (CBCT). This before-and-after observational study evaluated 30 patients (15 males and 15 females); mean age 35 years, who required CBCT. Exfoliating epithelial cells of the buccal mucosa were collected with a cotton swab immediately before and 10 days after CBCT. Following Papanicolaou staining, genotoxicity was evaluated by the micronucleus assay. The frequencies of pyknosis, karyorrhexis, karyolysis, budding and binucleation as cytotoxicity factors were also recorded. Expression of p53 was evaluated by IHC staining. Data were analyzed by paired samples t-test. Micronucleated cells, pyknosis, karyorrhexis, karyolysis, budding, cytotoxicity, and expression of p53 increased significantly after CBCT. CBCT exerts genotoxic and cytotoxic effects and leads to overexpression of p53 protein in the oral buccal mucosal cells.
本研究旨在评估成年人在接受锥形束 CT（CBCT）后口腔黏膜上皮的细胞毒性、遗传毒性和 p53 的免疫组织化学（IHC）表达。这是一项前后观察性研究，共评估了 30 名患者（15 名男性和 15 名女性）；平均年龄 35 岁，需要进行 CBCT。使用棉签在 CBCT 前和 10 天后立即收集颊黏膜的脱落上皮细胞。巴氏染色后，通过微核试验评估遗传毒性。还记录了核固缩、核碎裂、核溶解、芽生和双核的频率作为细胞毒性因素。通过免疫组织化学染色评估 p53 的表达。数据采用配对样本 t 检验进行分析。微核细胞、核固缩、核碎裂、核溶解、芽生、细胞毒性和 p53 的表达在 CBCT 后显著增加。CBCT 具有遗传毒性和细胞毒性作用，并导致口腔颊黏膜细胞中 p53 蛋白过度表达。
