Takacs L, Osawa H, Liszka K, Diamantstein T
Clin Exp Immunol. 1986 May;64(2):342-7.
The proportion and anatomical localization in murine thymus of T cell subpopulations, including those which are defined by the monoclonal anti-interleukin 2 (anti-IL-2) receptor antibody AMT-13, were studied by flow cytofluorometry and by immunohistochemical methods, both in irradiated and in normal mice. As a consequence of irradiation the proportion of AMT-13 positive cells and that of Lyt-1 positive cells were markedly enhanced, while the proportion of Lyt-2 positive cells was reduced. The vast majority of the AMT-13 positive cells both in normal and in irradiated thymi were located in the subcapsular area of the thymic cortex, whereas the irradiation resistant Lyt-1 positive cells were located in the medulla. These findings are compatible with the view that, similar to the developing thymus in the mouse embryo, in the regenerating adult thymus, AMT-13+ cells include the activated pro-thymocytes that repopulate the irradiated thymus.
采用流式细胞荧光术和免疫组织化学方法，对受照射小鼠和正常小鼠的T细胞亚群（包括那些由抗白细胞介素2（抗IL-2）受体单克隆抗体AMT-13所界定的亚群）在鼠胸腺中的比例及解剖定位进行了研究。照射后，AMT-13阳性细胞和Lyt-1阳性细胞的比例显著增加，而Lyt-2阳性细胞的比例则降低。正常胸腺和受照射胸腺中绝大多数AMT-13阳性细胞位于胸腺皮质的被膜下区域，而抗辐射的Lyt-1阳性细胞则位于髓质。这些发现支持这样一种观点，即与小鼠胚胎中发育的胸腺类似，在成年再生胸腺中，AMT-13+细胞包括那些使受照射胸腺重新细胞化的活化前胸腺细胞。
