Department of Occupational and Environmental Health, Key Laboratory of Environment and Health, Ministry of Education and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Rd, Wuhan, 430030, Hubei, China.
Department of Central Laboratory, the 5th Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Sci Rep. 2019 Mar 26;9(1):5121. doi: 10.1038/s41598-019-41577-4.
Cytokines play a critical role in the pathogenesis and development of cardiovascular diseases. However, data linking cytokines to risk and severity of acute coronary syndrome (ACS) are still limited. We measured plasma profile of 280 cytokines using a quantitative protein microarray in 12 ACS patients and 16 healthy controls, and identified 15 differentially expressed cytokines for ACS. Osteopontin, chemokine ligand 23, brain derived neurotrophic factor and C-reactive protein (CRP) were further validated using immunoassay in two independent case-control studies with a total of 210 ACS patients and 210 controls. We further examined their relations with incident ACS among 318 case-control pairs nested within the Dongfeng-Tongji cohort, and found plasma osteopontin and CRP concentrations were associated with incident ACS, and the multivariable-adjusted odds ratio (95% confidence interval) was 1.29 (1.06-1.57) per 1-SD increase for osteopontin and 1.30 (1.02-1.66) for CRP, respectively. Higher levels of circulating osteopontin were also correlated with higher severity of ACS, and earlier ACS onset time. Adding osteopontin alone or in combination with CRP modestly improved the predictive ability of ACS beyond the Framingham risk scores. Our findings suggested that osteopontin might be a biomarker for incident ACS, using osteopontin adds moderately to traditional cardiovascular risk factors.
细胞因子在心血管疾病的发病机制和发展中起着关键作用。然而，将细胞因子与急性冠状动脉综合征 (ACS) 的风险和严重程度联系起来的数据仍然有限。我们使用定量蛋白质微阵列在 12 名 ACS 患者和 16 名健康对照者中测量了 280 种细胞因子的血浆谱，并确定了 15 种与 ACS 相关的差异表达细胞因子。骨桥蛋白、趋化因子配体 23、脑源性神经营养因子和 C 反应蛋白 (CRP) 也使用免疫测定法在两个独立的病例对照研究中进行了验证，该研究共有 210 名 ACS 患者和 210 名对照者。我们进一步在东风-同济队列中 318 对病例对照中检查了它们与 ACS 事件的关系，发现血浆骨桥蛋白和 CRP 浓度与 ACS 事件相关，多变量调整的比值比（95%置信区间）分别为骨桥蛋白每增加 1-SD 的 1.29（1.06-1.57）和 CRP 的 1.30（1.02-1.66）。循环骨桥蛋白水平较高也与 ACS 的严重程度和 ACS 发病时间较早相关。单独或联合 CRP 升高可适度改善 ACS 预测能力，超越弗雷明汉风险评分。我们的研究结果表明，骨桥蛋白可能是 ACS 事件的生物标志物，使用骨桥蛋白可适度增加传统心血管危险因素。
