Department of Biological Science, Gachon University, Seongnam 13120, Korea.
Department of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, Korea.
Int J Mol Sci. 2019 Mar 26;20(6):1508. doi: 10.3390/ijms20061508.
Tomentosin is a natural sesquiterpene lactone extracted from various plants and is widely used as a medicine because it exhibits essential therapeutic properties. In this study, we investigated the anti-carcinogenic effects of tomentosin in human osteosarcoma MG-63 cells by performing cell migration/viability/proliferation, apoptosis, and reactive oxygen species (ROS) analysis assays. MG-63 cells were treated with various doses of tomentosin. After treatment with tomentosin, MG-63 cells were analyzed using the MTT assay, colony formation assay, cell counting assay, wound healing assay, Boyden chamber assay, zymography assay, cell cycle analysis, FITC Annexin V apoptosis assay, terminal deoxynucleotidyl transferase dUTP nick end labeling assay, western blot analysis, and ROS detection analysis. Our results indicated that tomentosin decreased cell viability and migration ability in MG-63 cells. Moreover, tomentosin induced apoptosis, cell cycle arrest, DNA damage, and ROS production in MG-63 cells. Furthermore, tomentosin-induced intracellular ROS decreased cell viability and induced apoptosis, cell cycle arrest, and DNA damage in MG-63 cells. Taken together, our results suggested that tomentosin exerted anti-carcinogenic effects in MG-63 cells by induction of intracellular ROS.
土木香内酯是一种从多种植物中提取的天然倍半萜内酯，由于具有重要的治疗特性，因此被广泛用作药物。在这项研究中，我们通过进行细胞迁移/活力/增殖、凋亡和活性氧（ROS）分析实验，研究了土木香内酯对人骨肉瘤 MG-63 细胞的抗癌作用。用不同剂量的土木香内酯处理 MG-63 细胞。用土木香内酯处理后，用 MTT 测定法、集落形成测定法、细胞计数测定法、划痕愈合测定法、Boyden 室测定法、明胶酶谱测定法、细胞周期分析、FITC Annexin V 凋亡测定法、末端脱氧核苷酸转移酶 dUTP 缺口末端标记测定法、western blot 分析和 ROS 检测分析来分析 MG-63 细胞。我们的结果表明，土木香内酯降低了 MG-63 细胞的活力和迁移能力。此外，土木香内酯诱导 MG-63 细胞凋亡、细胞周期停滞、DNA 损伤和 ROS 产生。此外，土木香内酯诱导的细胞内 ROS 降低了细胞活力并诱导 MG-63 细胞凋亡、细胞周期停滞和 DNA 损伤。总之，我们的结果表明，土木香内酯通过诱导细胞内 ROS 发挥了对 MG-63 细胞的抗癌作用。
