评估从昆虫病原线虫中分离出的两种细菌对某些害虫的致病性。
Assessing the Pathogenicity of Two Bacteria Isolated from the Entomopathogenic Nematode against and Some Pest Insects.
作者信息
Salgado-Morales Rosalba, Martínez-Ocampo Fernando, Obregón-Barboza Verónica, Vilchis-Martínez Kathia, Jiménez-Pérez Alfredo, Dantán-González Edgar
机构信息
Doctorado en Ciencias, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Chamilpa, 62209 Cuernavaca, Morelos, Mexico.
Laboratorio de Estudios Ecogenómicos, Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Chamilpa, 62209 Cuernavaca, Morelos, Mexico.
出版信息
Insects. 2019 Mar 26;10(3):83. doi: 10.3390/insects10030083.
The entomopathogenic nematodes are parasites of insects and are associated with mutualist symbiosis enterobacteria of the genus ; these bacteria are lethal to their host insects. MOR03 was isolated from sugarcane soil in Morelos state, Mexico. The molecular identification of the nematode was confirmed using sequences of the ITS1-5.8S-ITS2 region and the D2/D3 expansion segment of the 28S rRNA gene. In addition, two bacteria HIM3 and NA04 strains were isolated from the entomopathogenic nematode. The genomes of both bacteria were sequenced and assembled de novo. Phylogenetic analysis was confirmed by concatenated gene sequence datasets as HIM3 (16S rRNA, 23S rRNA, , , and genes) and NA04 (16S rRNA, 23S rRNA and genes). MOR03 infects , , , and larvae with LC50 values of 1.4, 23.5, 13.7, and 21.7 IJs/cm², respectively, at 48 h. These bacteria are pathogenic to various insects and have high injectable insecticide activity at 24 h.
昆虫病原线虫是昆虫的寄生虫，与 属的互利共生肠杆菌有关；这些细菌对其宿主昆虫具有致死性。MOR03 是从墨西哥莫雷洛斯州的甘蔗土壤中分离出来的。利用 ITS1-5.8S-ITS2 区域序列和 28S rRNA 基因的 D2/D3 扩展片段对该线虫进行了分子鉴定。此外，从昆虫病原线虫中分离出两种细菌 HIM3 和 NA04 菌株。对这两种细菌的基因组进行了测序并从头组装。通过串联基因序列数据集对 HIM3（16S rRNA、23S rRNA、 、 和 基因）和 NA04（16S rRNA、23S rRNA 和 基因）进行系统发育分析得到了证实。MOR03 在 48 小时时分别以 1.4、23.5、13.7 和 21.7 条感染性幼虫/平方厘米的 LC50 值感染 、 、 和 幼虫。这些细菌对多种昆虫具有致病性，并且在 24 小时时具有高注射杀虫剂活性。
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