从耐药基因预测革兰氏阴性杆菌的抗生素耐药性。
Predicting Antibiotic Resistance in Gram-Negative Bacilli from Resistance Genes.
机构信息
OpGen, Inc., Gaithersburg, Maryland, USA
OpGen, Inc., Gaithersburg, Maryland, USA.
出版信息
Antimicrob Agents Chemother. 2019 Mar 27;63(4). doi: 10.1128/AAC.02462-18. Print 2019 Apr.
Abstract
We developed a rapid high-throughput PCR test and evaluated highly antibiotic-resistant clinical isolates of ( = 2,919), ( = 1,974), ( = 1,150), and ( = 1,484) for several antibiotic resistance genes for comparison with phenotypic resistance across penicillins, cephalosporins, carbapenems, aminoglycosides, trimethoprim-sulfamethoxazole, fluoroquinolones, and macrolides. The isolates originated from hospitals in North America (34%), Europe (23%), Asia (13%), South America (12%), Africa (7%), or Oceania (1%) or were of unknown origin (9%). We developed statistical methods to predict phenotypic resistance from resistance genes for 49 antibiotic-organism combinations, including gentamicin, tobramycin, ciprofloxacin, levofloxacin, trimethoprim-sulfamethoxazole, ertapenem, imipenem, cefazolin, cefepime, cefotaxime, ceftazidime, ceftriaxone, ampicillin, and aztreonam. Average positive predictive values for genotypic prediction of phenotypic resistance were 91% for , 93% for , 87% for , and 92% for across the various antibiotics for this highly resistant cohort of bacterial isolates.
摘要
我们开发了一种快速高通量 PCR 检测方法，并评估了来自北美（34%）、欧洲（23%）、亚洲（13%）、南美（12%）、非洲（7%）或大洋洲（1%）或来源不明（9%）的 2919 株、1974 株、1150 株和 1484 株临床高度耐药分离株的多种抗生素耐药基因，以比较青霉素类、头孢菌素类、碳青霉烯类、氨基糖苷类、复方磺胺甲噁唑、氟喹诺酮类和大环内酯类药物的表型耐药性。我们开发了统计方法，以预测 49 种抗生素-生物体组合的表型耐药性，包括庆大霉素、妥布霉素、环丙沙星、左氧氟沙星、复方磺胺甲噁唑、厄他培南、亚胺培南、头孢唑林、头孢吡肟、头孢噻肟、头孢他啶、头孢曲松、氨苄西林和氨曲南。对于该高度耐药细菌分离株的各种抗生素，基因型预测表型耐药的平均阳性预测值分别为 91%、93%、87%和 92%。
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