Department of Animal Production, Faculty of Agriculture, Cairo University, Cairo, Egypt.
AgroParisTech, Paris, France.
PLoS One. 2021 Mar 15;16(3):e0248405. doi: 10.1371/journal.pone.0248405. eCollection 2021.
Gastrointestinal nematodes (GIN) are an important constraint in small ruminant production. Genetic selection for resistant animals is a potential sustainable control strategy. Advances in molecular genetics have led to the identification of several molecular genetic markers associated with genes affecting economic relevant traits. In this study, the variants in the genome of Creole goats resistant or susceptible to GIN were discovered from RNA-sequencing. We identified SNPs, insertions and deletions that distinguish the two genotypes, resistant and susceptible and we characterized these variants through functional analysis. The T cell receptor signalling pathway was one of the top significant pathways that distinguish the resistant from the susceptible genotype with 78% of the genes involved in this pathway showing genomic variants. These genomic variants are expected to provide useful resources especially for molecular breeding for GIN resistance in goats.
胃肠道线虫（GIN）是小反刍动物生产的一个重要限制因素。对抗性动物进行遗传选择是一种潜在的可持续控制策略。分子遗传学的进步导致了与影响经济相关性状的基因相关的几种分子遗传标记的鉴定。在这项研究中，从 RNA 测序中发现了对 GIN 具有抗性或敏感性的克里奥尔羊的基因组变异。我们鉴定了区分两种基因型（抗性和敏感型）的 SNP、插入和缺失，并通过功能分析对这些变异进行了表征。T 细胞受体信号通路是区分抗性和敏感基因型的最重要途径之一，该途径中 78%的基因显示出基因组变异。这些基因组变异有望提供有用的资源，特别是在针对 GIN 抗性的分子育种方面。
