Cooter M S, Eisma R J, Burleson J A, Leonard G, Lafreniere D, Kreutzer D L
Department of Surgery, University of Connecticut School of Medicine, Farmington 06030-3105, USA.
Laryngoscope. 1998 Jul;108(7):1066-70. doi: 10.1097/00005537-199807000-00021.
To characterize the existence and role of transforming growth factor-beta (TGF-beta) in otitis media with effusion (OME).
Retrospective.
The levels of two major TGF-beta isoforms, TGF-beta1 and TGF-beta2, in the middle ear effusions (MEEs) of 44 children were evaluated using enzyme-linked immunospecific assays (ELISAs). Forty-eight MEEs were separated into three clinically relevant groups (i.e., serous, mucoid, and purulent), and TGF-beta levels were correlated with clinical parameters of disease for these MEEs.
Both TGF-beta1 and TGF-beta2 were present in the samples. Mean levels of TGF-beta1 (920.36 +/- 437.75 pg/mg total protein) were generally 100-fold greater than those of TGF-beta2 (9.65 +/- 11.19 pg/mg total protein). TGF-beta1 levels were elevated in association with a history of previous tympanostomy tube placements (TTPs) (P = .029) and mucoid effusions (P = .042). TGF-beta2 levels were elevated in association with a history of previous TTPs (P = .100) and chronic (i.e., serous or mucoid) effusions (P = .003).
TGF-beta1 is present in the MEEs of children with OME. Furthermore, TGF-beta1 and TGF-beta2 levels were elevated differentially in the presence of chronic disease indicators in OME, suggesting that these isoforms may have differing roles in the inflammatory processes that characterize OME.
明确转化生长因子-β（TGF-β）在分泌性中耳炎（OME）中的存在情况及其作用。
回顾性研究。
采用酶联免疫特异性分析（ELISA）评估44例儿童中耳积液（MEE）中两种主要TGF-β亚型TGF-β1和TGF-β2的水平。将48份MEE分为三个临床相关组（即浆液性、黏液性和脓性），并将这些MEE的TGF-β水平与疾病的临床参数进行关联分析。
样本中同时存在TGF-β1和TGF-β2。TGF-β1的平均水平（920.36±437.75 pg/mg总蛋白）通常比TGF-β2（9.65±11.19 pg/mg总蛋白）高100倍。TGF-β1水平与既往鼓膜置管史（TTP）（P = 0.029）和黏液性积液（P = 0.042）相关而升高。TGF-β2水平与既往TTP史（P = 0.100）和慢性（即浆液性或黏液性）积液（P = 0.003）相关而升高。
TGF-β1存在于OME患儿的MEE中。此外，在OME存在慢性疾病指标时，TGF-β1和TGF-β2水平有差异地升高，提示这些亚型在OME特征性的炎症过程中可能具有不同作用。
