Strawa Jakub, Wajs-Bonikowska Anna, Leszczyńska Katarzyna, Ściepuk Małgorzata, Nazaruk Jolanta
a Department of Pharmacognosy , Medical University of Bialystok , Bialystok , Poland.
b Institute of General Food Chemistry , Lodz University of Technology , Lodz , Poland.
Nat Prod Res. 2016 Dec;30(23):2730-2733. doi: 10.1080/14786419.2016.1138303. Epub 2016 Jan 28.
The mixture of three phytosterols (campesterol, stigmasterol and β-sitosterol), β-sitosterol 3-O-glucoside and syringin were isolated from hexane and methanol extract of Cirsium rivulare roots after chromatographic separation. The main component of the source was syringin which was obtained with the yield 0.08% of the dry source. In hexane extract, the qualitative and quantitative composition of fatty acids was determined. The predominant component was linoleic acid (23.31 mg/g of extract). The extracts showed antioxidant activity. The ability to scavenge DPPH• free radical was in correlation with appointed total phenol content. The not-defatted methanolic extract was the most active. Hexane and defatted methanol extracts showed moderate antibacterial activity against G(+) and G(-) strains with MIC and MBC ranged from 25 to 200 μg/mL.
从溪蓟根的己烷和甲醇提取物中经色谱分离得到了三种植物甾醇（菜油甾醇、豆甾醇和β-谷甾醇）、β-谷甾醇3-O-葡萄糖苷和紫丁香苷的混合物。该原料的主要成分是紫丁香苷，其得率为原料干重的0.08%。测定了己烷提取物中脂肪酸的定性和定量组成。主要成分是亚油酸（23.31mg/g提取物）。提取物具有抗氧化活性。清除DPPH•自由基的能力与指定的总酚含量相关。未脱脂的甲醇提取物活性最强。己烷提取物和脱脂甲醇提取物对革兰氏阳性菌和革兰氏阴性菌菌株表现出中等抗菌活性，其最低抑菌浓度（MIC）和最低杀菌浓度（MBC）范围为25至200μg/mL。
