牛促卵泡激素β链前体cDNA的克隆与DNA序列分析
Cloning and DNA sequence analysis of the cDNA for the precursor of the beta chain of bovine follicle stimulating hormone.
作者信息
Esch F S, Mason A J, Cooksey K, Mercado M, Shimasaki S
出版信息
Proc Natl Acad Sci U S A. 1986 Sep;83(17):6618-21. doi: 10.1073/pnas.83.17.6618.
Follicle stimulating hormone (FSH) plays essential roles in the maintenance and development of oocytes and spermatozoa in normal reproductive physiology. FSH possesses two subunits, alpha and beta, the latter being responsible for FSH biological specificity. We have cloned and sequenced the cDNA encoding the FSH beta chain from a bovine anterior pituitary cDNA library. The mature molecule is 109 amino acids long and is preceded by a 20-amino acid putative signal peptide. RNA gel blot analysis of bovine pituitary RNA shows that the mRNA encoding beta chain of FSH is approximately 1.7 kilobases in length.
促卵泡激素（FSH）在正常生殖生理中对卵母细胞和精子的维持与发育起着至关重要的作用。FSH 由α和β两个亚基组成，其中β亚基决定了 FSH 的生物学特异性。我们从牛垂体前叶 cDNA 文库中克隆并测序了编码 FSHβ链的 cDNA。成熟分子由 109 个氨基酸组成，前面有一个 20 个氨基酸的假定信号肽。对牛垂体 RNA 的 RNA 凝胶印迹分析表明，编码 FSHβ链的 mRNA 长度约为 1.7 千碱基。
Zotero 插件