Department of Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Department of Biotechnology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Cell Tissue Res. 2019 Aug;377(2):215-227. doi: 10.1007/s00441-019-03015-9. Epub 2019 Mar 28.
Adipose stem cells (ASCs) are a great promise in wound healing due to their potential in differentiating into various cell lineages and secreting growth factors. The purpose of this study is to evaluate the in vivo effects of Aloe vera hydrogel loaded by allogeneic ASCs on a rat burn wound model. The ASCs were isolated, cultured and mixed with 50% Aloe vera hydrogel and injected intradermally around the wound. Demineralized bone matrix (DBM) was used as dressing in the experiment. The burn wound-healing properties of different experimental groups were investigated by histopathological, molecular, scanning electron microscopic and biochemical analysis at the 7th, 14th and 28th days post-wounding. The Aloe vera and DBM-Aloe vera groups showed almost similar healing properties, while treatment by DBM-Aloe vera/ASCs significantly enhanced wound healing. The levels of transforming growth factor-β1 (TGF-β1) and interleukin-1β markedly decreased at the 7th day post-injury, in the DBM-Aloe vera/ASC-treated group, suggesting that this treatment regime subsided the inflammatory responses. Angiogenesis, re-epithelialization and the level of TGF-β1 in the wounds treated with DBM-Aloe vera/ASCs were also remarkably higher than those of other groups, at the 14th day post-injury. Besides, scar formation significantly decreased in the DBM-Aloe vera/ASC-treated wounds when compared with other groups. Our biochemical results were in agreement with the molecular and histopathological findings and strongly demonstrated that a DBM-Aloe vera/ASC composite can stimulate burn wound healing. These results suggest that the DBM-Aloe vera/ASC composite can be considered as a promising therapeutic strategy in the treatment of burn wounds.
脂肪干细胞（ASCs）在伤口愈合中具有巨大的潜力，因为它们可以分化为多种细胞谱系并分泌生长因子。本研究旨在评估负载异体 ASC 的库拉索芦荟水凝胶对大鼠烧伤创面模型的体内作用。ASCs 被分离、培养并与 50%的库拉索芦荟水凝胶混合，然后皮内注射到伤口周围。脱矿骨基质（DBM）被用作实验中的敷料。通过组织病理学、分子、扫描电子显微镜和生化分析，在创伤后第 7、14 和 28 天研究了不同实验组的烧伤伤口愈合特性。库拉索芦荟和 DBM-库拉索芦荟组显示出几乎相似的愈合特性，而 DBM-库拉索芦荟/ASC 治疗显著增强了伤口愈合。在 DBM-库拉索芦荟/ASC 治疗组中，转化生长因子-β1（TGF-β1）和白细胞介素-1β的水平在创伤后第 7 天显著降低，表明这种治疗方案减轻了炎症反应。在创伤后第 14 天，DBM-库拉索芦荟/ASC 治疗的伤口中的血管生成、再上皮化和 TGF-β1 水平也明显高于其他组。此外，与其他组相比，DBM-库拉索芦荟/ASC 治疗的伤口中的瘢痕形成明显减少。我们的生化结果与分子和组织病理学发现一致，强烈表明 DBM-库拉索芦荟/ASC 复合材料可以刺激烧伤伤口愈合。这些结果表明，DBM-库拉索芦荟/ASC 复合材料可被视为治疗烧伤的一种有前途的治疗策略。
