Shahmohammadi Azin, Samadian Hadi, Heidari Keshel Saeed, Rashidi Khodabakhsh, Kiani Amir, Soleimani Masoud, Goudarzi Farjam
Department of Applied Cell Sciences and Tissue Engineering, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran .
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Bioimpacts. 2024;14(5):30193. doi: 10.34172/bi.2024.30193. Epub 2024 Jan 21.
Wound healing is a major therapeutic concern in regenerative medicine. The current study aimed to investigate the second-degree burn wound treatment in rats using rat adipose- derived stem cells (ADSCs) and manganese nanoparticles (MnO-NPs) in a polycaprolactone/gelatin electrospun nanofiber scaffold.
After the synthesis of nanoparticles and electrospinning of nanofibers, the SEM analysis, contact angle, mechanical strength, blood compatibility, porosity, swelling, biodegradability, cell viability, and adhesion assays were performed. According to the results, the PCL/Gel/5%MnO-NPs nanofiber (Mn-5%) was determined to be the most suitable scaffold. The ADSCs-seeded Mn-5% scaffolds were applied as a burn wound dressing. The wound closure rate, IL-1β, and IL-6 level, hydroxyproline, and glycosaminoglycans content were measured, and the hematoxylin and eosin, Masson's trichrome, and immunohistochemistry stainings were carried out.
Based on the results, in Mn+S (ADSCs+PCL/Gel/5%MnO-NPs nanofiber) and N+S (ADSCs+PCL/Gel nanofiber) groups, the IL-6 and IL-1β levels were reduced, and the percentage of wound closure, glycosaminoglycans, and hydroxyproline content were increased compared to the control group (<0.05). Also, the lowest amount of α-SMA was observed in these two groups, demonstrating stem cells' role in reducing α-SMA levels and thus preventing fibrosis. Moreover, the amount of α-SMA in the Mn+S group is lower than in the N+S group and, is closer to healthy skin. According to histology results, the best type of treatment was observed in the Mn+S group.
In conclusion, the ADSCs-seeded PCL/Gel/5%MnO-NPs scaffold demonstrated considerable therapeutic effects in burn wound healing.
伤口愈合是再生医学中的一个主要治疗关注点。当前研究旨在探讨在聚己内酯/明胶电纺纳米纤维支架中使用大鼠脂肪来源干细胞（ADSCs）和锰纳米颗粒（MnO-NPs）对大鼠二度烧伤创面的治疗效果。
在纳米颗粒合成和纳米纤维电纺之后，进行扫描电子显微镜分析、接触角、机械强度、血液相容性、孔隙率、膨胀性、生物降解性、细胞活力和粘附试验。根据结果，确定PCL/Gel/5%MnO-NPs纳米纤维（Mn-5%）为最合适的支架。接种ADSCs的Mn-5%支架用作烧伤创面敷料。测量伤口闭合率、白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）水平、羟脯氨酸和糖胺聚糖含量，并进行苏木精和伊红染色、Masson三色染色和免疫组织化学染色。
基于结果，与对照组相比，在Mn+S（ADSCs+PCL/Gel/5%MnO-NPs纳米纤维）和N+S（ADSCs+PCL/Gel纳米纤维）组中，IL-6和IL-1β水平降低，伤口闭合百分比、糖胺聚糖和羟脯氨酸含量增加（<0.05）。此外，在这两组中观察到α-平滑肌肌动蛋白（α-SMA）的量最低，表明干细胞在降低α-SMA水平从而预防纤维化方面的作用。此外，Mn+S组中的α-SMA量低于N+S组，且更接近健康皮肤。根据组织学结果，在Mn+S组中观察到最佳治疗类型。
总之，接种ADSCs的PCL/Gel/5%MnO-NPs支架在烧伤创面愈合中显示出显著的治疗效果。
