Yang Yixia, Hou Jingzhou, Huo Danqun, Wang Xianfeng, Li Jiawei, Xu Guoli, Bian Minghong, He Qiang, Hou Changjun, Yang Mei
Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing, 400044, People's Republic of China.
Key Laboratory of Eco-Environment of Three Gorges Region of Ministry of Education, Faculty of Urban Construction and Environmental Engineering, Chongqing University, Chongqing, 400045, China.
Mikrochim Acta. 2019 Mar 28;186(4):259. doi: 10.1007/s00604-019-3361-5.
A fluorometric method was developed for the determination of the insecticide cartap. It is based on the use of green emitting carbon dots (CDs) and gold nanoparticles (Au NPs). The CDs were prepared from phenol and ethylene diamine by a hydrothermal route. They have excitation/emission maxima at 410/513 nm) and a fluorescence quantum yield of 29%. They were characterized by TEM, Raman, XRD, XPS, FT-IR, UV and fluorescence spectroscopies. The green fluorescence of the CDs is strongly reduced by the red-colored Au NPs because of an inner filter effect. Upon addition of cartap, it will cause the aggregation of the Au NPs owing to Au-N interaction between Au NPs and cartap to form purple colored aggregates with spectra that do not overlap the green emission of the CDs. Hence, their fluorescence is restored. Under optimum conditions, the method allows for the quantitation of cartap in the 5-300 nM concentration range, and the detection limit is 3.8 nM. The method was successfully applied to the determination of cartap in spiked real samples and gave satisfactory results. Graphical abstract Schematic presentation of green emitting carbon dots for sensitive fluorometric determination of cartap based on its aggregation effect on gold nanoparticles.
开发了一种用于测定杀虫剂杀螟丹的荧光方法。该方法基于使用发射绿色荧光的碳点（CDs）和金纳米颗粒（Au NPs）。通过水热法由苯酚和乙二胺制备得到碳点。它们的激发/发射最大值在410/513nm，荧光量子产率为29%。通过透射电子显微镜（TEM）、拉曼光谱、X射线衍射（XRD）、X射线光电子能谱（XPS）、傅里叶变换红外光谱（FT-IR）、紫外光谱和荧光光谱对其进行了表征。由于内滤效应，红色的金纳米颗粒会使碳点的绿色荧光强烈猝灭。加入杀螟丹后，由于金纳米颗粒与杀螟丹之间的金-氮相互作用，会导致金纳米颗粒聚集形成紫色聚集体，其光谱与碳点的绿色发射光谱不重叠。因此，碳点的荧光得以恢复。在最佳条件下，该方法可对浓度范围为5-300 nM的杀螟丹进行定量分析，检测限为3.8 nM。该方法成功应用于加标实际样品中杀螟丹的测定，并获得了满意的结果。图形摘要基于杀螟丹对金纳米颗粒的聚集效应，用于灵敏荧光测定杀螟丹的发射绿色荧光的碳点的示意图。
