Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan Province, 650500, China.
Department of Civil and environmental engineering, Carnegie Mellon University, Pittsburgh, PA, 15213, USA.
Mikrochim Acta. 2018 Jan 27;185(2):137. doi: 10.1007/s00604-018-2685-x.
The authors describe a method for the determination of norfloxacin (NOR) or ciprofloxacin (CIP). It is making use of a combination of fluorescence enhancement and magnetic solid-phase extraction (MSPE). Sulfur-doped carbon dots (S-CDs) are used as a fluorescent probe. They were prepared by a one-pot method using poly(4-styrenesulfonic acid-co-maleic acid) (PSMA) as a source for carbon and sulfur. NOR or CIP act as sensitizers of fluorescence (with excitation/emission maxima at 324/412 nm), probably due to strong hydrogen bond interaction and charge transfer with the S-CDs. The S-CDs were characterized by using TEM, XRD, XPS, FT-IR, UV-Vis and fluorescence spectroscopies. Response is linear in the 0.02-1.25 μM NOR concentration range, and the detection limit is 4.6 nM. The respective data for CIP are 0.02-1.0 μM and 6.7 nM. The average recoveries of NOR and CIP residues from spiked bovine raw milk are 96.2%105.2% and 92.3%102.5%. Graphical abstract Sulfur doped carbon dots (S-CDs) were synthesized by a hydrothermal method using poly(4-styrenesulfonic acid-co-maleic acid) (PSMA). Norfloxacin (NOR) or ciprofloxacin (CIP) was extracted by magnetic nanoparticles (MNPs), they were detected by carbon dots fluorescence enhancement.
作者描述了一种测定诺氟沙星（NOR）或环丙沙星（CIP）的方法。该方法结合了荧光增强和磁性固相萃取（MSPE）。硫掺杂碳点（S-CDs）用作荧光探针。它们是通过一锅法使用聚（4-苯乙烯磺酸-co-马来酸）（PSMA）作为碳和硫的来源制备的。NOR 或 CIP 作为荧光敏化剂（激发/发射最大值为 324/412nm），可能是由于与 S-CDs 之间存在强氢键相互作用和电荷转移。通过 TEM、XRD、XPS、FT-IR、UV-Vis 和荧光光谱对 S-CDs 进行了表征。在 0.02-1.25μM NOR 浓度范围内，响应呈线性，检测限为 4.6nM。CIP 的相应数据为 0.02-1.0μM 和 6.7nM。从添加牛生乳中的 NOR 和 CIP 残留的平均回收率分别为 96.2%-105.2%和 92.3%-102.5%。 图表摘要 采用水热法以聚（4-苯乙烯磺酸-co-马来酸）（PSMA）为原料合成了硫掺杂碳点（S-CDs）。磁性纳米粒子（MNPs）萃取诺氟沙星（NOR）或环丙沙星（CIP），通过碳点荧光增强检测。
