Key Laboratory of Sustainable Development of Polar Fishery, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 266071, China.
Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, School of Marine Sciences, Sun Yat-Sen University, Guangzhou, Guangdong 510006, China.
Molecules. 2019 Mar 28;24(7):1228. doi: 10.3390/molecules24071228.
Cane molasses is one of the main by-products of sugar refineries, which is rich in sucrose. In this work, low-cost cane molasses was introduced as an alternative substrate for isomaltulose production. Using the engineered lipolytica, the isomaltulose production reached the highest (102.6 g L¹) at flask level with pretreated cane molasses of 350 g L¹ and corn steep liquor of 1.0 g L¹. During fed-batch fermentation, the maximal isomaltulose concentration (161.2 g L¹) was achieved with 0.96 g g¹ yield within 80 h. Simultaneously, monosaccharides were completely depleted, harvesting the high isomaltulose purity (97.4%) and high lipid level (12.2 g L¹). Additionally, the lipids comprised of 94.29% C and C fatty acids, were proved suitable for biodiesel production. Therefore, the bioprocess employed using cane molasses in this study was low-cost and eco-friendly for high-purity isomaltulose production, coupling with valuable lipids.
甘蔗糖蜜是制糖厂的主要副产物之一，富含蔗糖。在这项工作中，我们将低成本的甘蔗糖蜜作为异麦芽酮糖生产的替代底物。利用工程化的酵母，在预处理的甘蔗糖蜜为 350 g/L 和玉米浆为 1.0 g/L 的条件下，摇瓶水平的异麦芽酮糖产量最高（102.6 g/L）。在分批补料发酵过程中，以 0.96 g/g 的产率在 80 h 内达到了最高的异麦芽酮糖浓度（161.2 g/L）。同时，单糖被完全耗尽，收获了高纯度（97.4%）和高油脂水平（12.2 g/L）的异麦芽酮糖。此外，所得到的油脂由 94.29%的 C 和 C 脂肪酸组成，证明适合用于生物柴油生产。因此，本研究中使用甘蔗糖蜜的生物工艺用于生产高纯度异麦芽酮糖具有低成本和环境友好的特点，同时还联产有价值的油脂。
