Jilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun 130024, PR China.
Jilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun 130024, PR China.
Carbohydr Polym. 2019 Jun 15;214:34-43. doi: 10.1016/j.carbpol.2019.03.002. Epub 2019 Mar 4.
A novel polysaccharide (WCCP-N-b) with a molecular weight of 18 kDa was isolated and purified from the fruiting bodies of Cantharellus cibarius. Monosaccharide composition, methylation analysis and NMR spectra indicated that WCCP-N-b was a linear α-1,6-galactan, partially methylated at O-3 of galactose. The molar ratio of Gal, 3-methylated-Gal, Glc and Man was 14.4:4.6:1.0:1.2. WCCP-N-b could significantly increase macrophage phagocytosis, release of NO and secretion of TNF-α, IL-6 and IL-1β. On a cellular mechanistic level, WCCP-N-b activated MAPKs and NF-κB signaling pathway via Toll-like receptor 2 (TLR2). To further elucidate the structure-function relationship, WCCP-N-b was hydrolyzed by acid. Four degraded fragments were obtained, with molecular weights of 16.1 kDa, 11.2 kDa, 5 kDa and 3.5 kDa, respectively. Their macrophage activation effects were significantly decreased along with the molecular weight decrease. Collectively, WCCP-N-b could activate RAW264.7 cells, and the activation effect was related to its molecular weight.
从鸡油菌子实体中分离纯化得到一种分子量为 18 kDa 的新型多糖（WCCP-N-b）。单糖组成、甲基化分析和 NMR 谱表明，WCCP-N-b 是一种线性 α-1,6-半乳糖，部分在半乳糖的 O-3 位被甲基化。Gal、3-甲基化-Gal、Glc 和 Man 的摩尔比为 14.4:4.6:1.0:1.2。WCCP-N-b 能显著增强巨噬细胞的吞噬作用，促进 NO 和 TNF-α、IL-6 和 IL-1β的分泌。在细胞机制水平上，WCCP-N-b 通过 Toll 样受体 2（TLR2）激活 MAPKs 和 NF-κB 信号通路。为了进一步阐明结构-功能关系，用酸水解 WCCP-N-b。得到四个降解片段，分子量分别为 16.1 kDa、11.2 kDa、5 kDa 和 3.5 kDa。随着分子量的降低，它们对巨噬细胞的激活作用显著降低。总之，WCCP-N-b 可以激活 RAW264.7 细胞，其激活作用与其分子量有关。
