Biotechnological Institute of Chinese Materia Medica, College of Pharmacy, Jinan University, 601 Huangpu Avenue West, Guangzhou 510632, China; School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Department of Pharmacology, Jinan University, 601 Huangpu Avenue West, Guangzhou 510632, China.
Int J Biol Macromol. 2021 Jul 31;183:145-157. doi: 10.1016/j.ijbiomac.2021.04.084. Epub 2021 Apr 18.
Two novel glucans named MIPB50-W and MIPB50-S-1 were obtained from edible Morchella importuna with molecular weights (M) of 939.2 kDa and 444.5 kDa, respectively. MIPB50-W has a backbone of α-(1 → 4)-d-glucan, which was substituted at O-6 position by α-d-Glcp-(1→. Moreover, MIPB50-S-1 has a backbone of α-(1 → 4)-d-glucan, which was substituted at O-6 position by α-d-Glcp-(1 → 6)-α-d-Glcp-(1→. This is the first report about glucan found in Morchella mushrooms. Furthermore, MIPB50-W and MIPB50-S-1 strengthened the phagocytosis function and the promoted secretion of interleukins (IL)-6/tumor necrosis factor-alpha (TNF-α) and nitric oxide (NO), which induced the activation of Toll-like receptor 2 (TLR2), TLR4 as well as mitogen activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) pathways. Interestingly, MIPB50-S-1 performed the better immunomodulatory activity than that of MIPB50-W in almost all tests. Therefore, MIPB50-W and MIPB50-S-1 are potential immune-enhancing components of functional foods.
从可食用的羊肚菌中获得了两种新型葡聚糖，分别命名为 MIPB50-W 和 MIPB50-S-1，它们的分子量（M）分别为 939.2 kDa 和 444.5 kDa。MIPB50-W 的主链为α-(1 → 4)-d-葡聚糖，在 O-6 位被α-d-Glcp-(1→取代。此外，MIPB50-S-1 的主链为α-(1 → 4)-d-葡聚糖，在 O-6 位被α-d-Glcp-(1 → 6)-α-d-Glcp-(1→取代。这是首次在羊肚菌中发现葡聚糖的报道。此外，MIPB50-W 和 MIPB50-S-1 增强了吞噬功能，并促进白细胞介素（IL）-6/肿瘤坏死因子-α（TNF-α）和一氧化氮（NO）的分泌，从而激活 Toll 样受体 2（TLR2）、TLR4 以及丝裂原活化蛋白激酶（MAPK）和核因子-κB（NF-κB）途径。有趣的是，在几乎所有测试中，MIPB50-S-1 的免疫调节活性均优于 MIPB50-W。因此，MIPB50-W 和 MIPB50-S-1 是功能性食品中潜在的免疫增强成分。
