Engineering Research Center of Glycoconjugates Ministry of Education, Jilin Provincial Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun, Jilin 130024, P.R. China.
Int J Mol Med. 2021 Apr;47(4). doi: 10.3892/ijmm.2021.4883. Epub 2021 Feb 12.
The present study was designed to investigate the structure and immunomodulatory activity of a polysaccharide. A novel acidic β‑glucan (WCCP‑A‑b; molecular weight, 7.3 kDa) was purified from the fruiting bodies of the edible mushroom , which possesses high nutritional values. WCCP‑A‑b was composed primarily of glucose (89.7%) and glucuronic acid (8.8%). Methylation and nuclear magnetic resonance analysis suggested that WCCP‑A‑b contained β‑D‑1,6‑glucan as its main chain, which was substituted at O‑3 by β‑1,3‑D‑Glc oligosaccharides or a single‑unit of β‑Glc residues. Minor β‑1,4‑D‑GlcA residues may also be present in the side chains. The degree of branching was ~20.9%. Moreover, WCCP‑A‑b possessed a macrophage activating effect by promoting the secretion of nitric oxide, TNF‑α and IL‑6 in a dose‑dependent manner. At a cellular mechanistic level, WCCP‑A‑b activated macrophages via the MAPK signaling pathway. The present results provided useful information for supporting further investigations on the structure‑activity association of polysaccharides from , and indicated that the novel β‑glucan may be a potent natural immunomodulator, thus promoting the application of as a valuable source for functional food.
本研究旨在研究一种多糖的结构和免疫调节活性。从食用蘑菇 的子实体中纯化出一种新型酸性 β-葡聚糖（WCCP-A-b；分子量 7.3 kDa），具有较高的营养价值。WCCP-A-b 主要由葡萄糖（89.7%）和葡萄糖醛酸（8.8%）组成。甲基化和核磁共振分析表明，WCCP-A-b 含有 β-D-1,6-葡聚糖作为其主链，在 O-3 位被 β-1,3-D-Glc 低聚糖或单个 β-Glc 残基取代。侧链中可能还存在少量的 β-1,4-D-GlcA 残基。支化度约为 20.9%。此外，WCCP-A-b 通过促进一氧化氮、TNF-α 和 IL-6 的分泌以剂量依赖的方式发挥巨噬细胞激活作用。在细胞机制水平上，WCCP-A-b 通过 MAPK 信号通路激活巨噬细胞。本研究结果为进一步研究 多糖的结构-活性关系提供了有用信息，并表明新型 β-葡聚糖可能是一种有效的天然免疫调节剂，从而促进了 将其作为功能性食品的有价值来源的应用。
