School of Materials Science and Engineering, Ocean University of China, Songling Road 238, Qingdao, 266100, PR China; College of Materials Science and Chemical Engineering, Harbin Engineering University, Harbin, 150001, PR China; State Key Laboratory for Marine Corrosion and Protection, Luoyang Ship Material Research Institute, Qingdao, 266237, PR China.
School of Materials Science and Engineering, Ocean University of China, Songling Road 238, Qingdao, 266100, PR China.
Anal Chim Acta. 2019 Jul 11;1061:161-168. doi: 10.1016/j.aca.2019.02.028. Epub 2019 Feb 25.
A spiropyran derivative containing an ethyl group (SPEt) was applied for fluorescent recognition of Cu. With Cu addition, the SPEt-contained solution using a mixed solvent of DMF/HO (9/1, v/v) showed a very strong characteristic emission peak with high quantum yield which is 4.3 times that of the pure SPEt solution. As a result, Cu was successfully picked out from totally 17 kinds of common metal ions and a detection limit as low as 14.9 fM had been achieved in using this assay system. Particularly, the recognition process was temperature- and time-dependent and could be effectively promoted by heating. In addition, SPEt had been used to image and detect Cu in mussels. Thus, our results in this study provided an exclusive Cu recognition via fluorometry in wide temperature, time, and dynamic ranges of assay, enabled a femtomolar scale detection of Cu by spiropyran derivative, and showed a promising imaging ability of Cu in marine living organisms.
一种含有乙基基团的螺吡喃衍生物（SPEt）被用于荧光识别 Cu。加入 Cu 后，在 DMF/HO（9/1，v/v）混合溶剂中使用的含 SPEt 的溶液显示出非常强的特征发射峰，量子产率高达纯 SPEt 溶液的 4.3 倍。因此，Cu 可以从总共 17 种常见金属离子中成功地被挑出，在使用该检测体系时检测限低至 14.9 fM。特别是，识别过程是温度和时间依赖性的，可以通过加热有效地促进。此外，SPEt 已被用于贻贝中 Cu 的成像和检测。因此，我们在这项研究中的结果提供了一种通过荧光法在广泛的测定温度、时间和动态范围内进行独特的 Cu 识别，通过螺吡喃衍生物实现了 femtomolar 级别的 Cu 检测，并显示了在海洋生物中 Cu 的有前景的成像能力。
