Department of Food Engineering, School of Animal Science and Food Engineering, University of São Paulo, Av. Duque de Caxias Norte, 225, CEP 13635-900, Pirassununga, SP, Brazil.
Department of Biochemistry and Immunology, School of Medicine at Ribeirão Preto, University of São Paulo, CEP 14040-903, Ribeirão Preto, SP, Brazil.
Food Chem Toxicol. 2019 Jun;128:21-34. doi: 10.1016/j.fct.2019.03.046. Epub 2019 Mar 27.
This study aimed to assess the exposure of Brazilian residents (N = 86) from rural areas to multiple mycotoxins and characterize the associated risk in two sampling periods (SP) (April-May and December/2016). Mycotoxins in food and urine samples were determined by liquid chromatography coupled to tandem mass spectrometry. Mean probable daily intake (PDI) values based on occurrence data in foods in both SP varied from 0.007 to 0.013, 0.069 to 1.002, 0.119 to 0.321 and 0.013-0.156 μg kg body weight (bw) day for aflatoxins (AFs), deoxynivalenol (DON), fumonisins (FBs) and zearalenone (ZEN), respectively. Mean PDI values based on urinary biomarkers were 0.001, 84.914, 0.031, 0.377 and 0.002 μg kg bw day for AFB, DON, ochratoxin A (OTA), FB and ZEN, respectively. Hazard quotient (HQ) calculated using food data revealed a potential health concern for ZEN in 2nd SP. HQ > 1 based on urinary biomarkers were observed for DON in the two SP. Although OTA was not detected in any food sample, the HQ based on urinary OTA levels was >1 in the 1st SP. Margin of exposure values for AF from food and urine data in the 1st SP were below 10,000, indicating potential health risks.
本研究旨在评估巴西农村居民（N=86）暴露于多种霉菌毒素的情况，并在两个采样期（SP）（2016 年 4 月至 5 月和 12 月/2016 年）对相关风险进行特征描述。通过液相色谱-串联质谱法测定食品和尿液样本中的霉菌毒素。基于两个 SP 中食品中发生数据的平均可能每日摄入量（PDI）值，在 0.007 到 0.013、0.069 到 1.002、0.119 到 0.321 和 0.013 到 0.156μgkg 体重（bw）天之间不等，分别为黄曲霉毒素（AFs）、脱氧雪腐镰刀菌烯醇（DON）、伏马菌素（FBs）和玉米赤霉烯酮（ZEN）。基于尿液生物标志物的平均 PDI 值分别为 0.001、84.914、0.031、0.377 和 0.002μgkg bw 天，分别为 AFB、DON、赭曲霉毒素 A（OTA）、FB 和 ZEN。使用食品数据计算的危害商数（HQ）表明，SP2 中 ZEN 可能对健康造成威胁。在两个 SP 中，基于尿液生物标志物的 HQ 均大于 1。尽管在任何食品样本中均未检测到 OTA，但在 SP1 中基于尿液 OTA 水平的 HQ 大于 1。SP1 中食品和尿液数据中来自 AF 的暴露边际值低于 10,000，表明存在潜在的健康风险。
